Marina V. Pakhomova

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Two modes of phloem loading have been proposed, apoplastic and symplastic, depending on the structure of sieve element-companion cell complexes (SE-CCCs) in minor vein phloem. Species are usually classified as either apoplastic or symplastic loaders although the cytology of SE-CCCs in minor veins of the majority of plants indicates that both mechanisms can(More)
The discovery of abundant plasmodesmata at the bundle sheath/phloem interface in Oleaceae (Gamalei, 1974) and Cucurbitaceae (Turgeon et al., 1975) raised the questions as to whether these plasmodesmata are functional in phloem loading and how widespread symplasmic loading would be. Analysis of over 800 dicot species allowed the definition of "open" and(More)
The phloem-loading-related effects of temperature on leaf ultrastructure were studied in seven species having numerous plasmodesmatal connections between the mesophyll and phloem (symplasmic minor-vein configuration). The response to temperature (between 5 and 30 °C) was characterized by drastic changes in the endoplasmic-reticulum labyrinth (ER labyrinth)(More)
Ribosomes and rRNAs were isolated from cells of green-blue alga Anabaena variabilis. The sedimentation properties of the ribosomes as well as density, molecular weights and nucleotide composition of rRNAs were determined. The ribosomes were found to have the S20,w value equal to 67.2+/-0.4S, whereas those of the ribosome subunits were 48.6+/-0.8 and(More)
The methods of extracting total protein from the biomass of Spirulina platensis are discussed. The results of studying different procedures of cell wall disruption, soluble protein extraction and precipitation are presented. The best results can be obtained using mechanical disintegration of Spirulina cells for 20 min (at a temperature not higher than 25(More)
A database containing information on the trait complex for terminal phloem of dicots leaf having been created during the last thirty years was analyzed. The typology of terminal phloem was verified. The monotypy of plant taxa up to the family level was confirmed. For orders and subclasses, the wide distribution of structural parallelisms was demonstrated.(More)