Marina V. Pakhomova

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Two modes of phloem loading have been proposed, apoplastic and symplastic, depending on the structure of sieve element-companion cell complexes (SE-CCCs) in minor vein phloem. Species are usually classified as either apoplastic or symplastic loaders although the cytology of SE-CCCs in minor veins of the majority of plants indicates that both mechanisms can(More)
The phloem-loading-related effects of temperature on leaf ultrastructure were studied in seven species having numerous plasmodesmatal connections between the mesophyll and phloem (symplasmic minor-vein configuration). The response to temperature (between 5 and 30 °C) was characterized by drastic changes in the endoplasmic-reticulum labyrinth (ER labyrinth)(More)
We performed electron-microscopic examination of structural diurnal changes in the lumen of sieve tubes and the vacuolar system of corresponding companion cells and changes induced by the experimental blockage of assimilate export from the leaf by its cold-girdling. For these investigations, Cucurbita pepo L. and Helianthus annuus L. plants were used, that(More)
The discovery of abundant plasmodesmata at the bundle sheath/phloem interface in Oleaceae (Gamalei, 1974) and Cucurbitaceae (Turgeon et al., 1975) raised the questions as to whether these plasmodesmata are functional in phloem loading and how widespread symplasmic loading would be. Analysis of over 800 dicot species allowed the definition of "open" and(More)
364 Structural and functional evolution of the terminal phloem of dicotyledonous plants became a subject of active investigation after detection and description of several types of its terminal complexes differing in the structure of the sieve-tube companion cells: intermediary cells [1, 2], transfer cells [3, 4], and ordinary cells [5, 6]. In these first(More)
The methods of extracting total protein from the biomass of Spirulina platensis are discussed. The results of studying different procedures of cell wall disruption, soluble protein extraction and precipitation are presented. The best results can be obtained using mechanical disintegration of Spirulina cells for 20 min (at a temperature not higher than 25(More)