Marina V. Padkina

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Pichia pastoris is a methylotrophic yeast that is widely used for the expression of heterologous proteins. Here, we have investigated the dependence of AOX1 and CAT1 expression on the source of nitrogen. It has shown that the expression of AOX1 and CAT1 depends on the source of nitrogen. In the presence of a rich nitrogen source, such as glutamine or(More)
The inbreed line of Nicotiana tabacum L. plants harboring a heterologous gene of bovine interferon-gamma sIFNG under the control of constitutive 35S CaMV promoter was created by Agrobacterium-mediated genetic transformation. The transformation of leaf discs yielded six independent transgenic plants (T0 generation). All six transformants demonstrated(More)
Genes encoding truncated versions of bovine and chicken gamma interferon genes lacking predicted protease cleavage sites at the C-terminus were constructed and expressed in the yeast Pichia pastoris. The recombinant proteins possessed increased stability in comparison with the corresponding wild-type gamma interferons while retaining biological activity.(More)
The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs’ and RNA-binding proteins’ interaction in post-transcriptional(More)
Methylotrophic yeast Pichia pastoris has proved to be especially useful for production of various heterologous proteins. In biotechnology it is very important to maintain the balance between high levels of heterologous gene expression and cell viability. Decisive understanding of gene regulation mechanisms is essential for reaching this goal. In this study,(More)
Conditions have been created for the isolation of the chimeric protein Alburon16 (human albumin-interferon-α 16) from a culture medium of the yeast Pichia pastoris, under which there is no aggregation of the protein and its biological activity is maintained. The proposed scheme can be used for the isolation and purification of a chimeric protein in(More)
Acid phosphatases of budding yeast have been studied for more than forty years. This paper covers biochemical characteristics of acid phosphatases and different aspects in expression regulation of eukaryotic genes, which were researched using acid phosphatases model. A special focus is devoted to cyclin-dependent kinase Pho85p, a negative transcriptional(More)
Evolution of multigene families are considered in the review on the example of the PHO gene family encoding the structure of acid phosphatases in the yeast Saccharomyces cerevisiae. Analysis of the databases demonstrated that divergence due to a change in regulation of the structural genes and their inclusion in new regulatory networks is the main direction(More)
In the present study, we constructed a Pichia pastoris mutant strain PS111 that lacks one member of the yapsin family through disruption of the YPS1 gene coding for aspartic protease yapsin 1. Under normal growth conditions, the PS111 mutant strain did not show detectable growth defects. Unlike the S. cerevisiae yps1 mutant, the P. pastoris PS111 strain(More)
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