Marina V Lysova

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The TATA-binding protein (TBP) is the subunit of basal transcription factor TFIID that recognizes the TATA boxes of TATA-containing promoters in class II genes, binds to them, and starts the assemblage of the RNA polymerase II basal transcription complex. The sequence of the TATA box with its flanking regions affects the levels of basal and activated(More)
TATA-binding protein (TBP) is the first basal factor that recognizes and binds a TATA box on TATA-containing gene promoters transcribed by RNA polymerase II. Data available in the literature are indicative of admissible variability of the TATA box. The TATA box flanking sequences can influence TBP affinity as well as the level of basal and activated(More)
We have analyzed an interaction of the general transcription complex RNA polymerase II proteins (RNA polymerase II, factors TBP, TFIIB, TFIIF, TFIIE and TFIIH) S. cerevisiae with the oligoribonucleotides. With the help of method EMSA was shown that labeled 32P labeled oligoribonucleotide 5'-ACUCUCUUCCGCAUCGC-3' (r-17) binds with the proteins and generates(More)
Lac(+)/Lac(-) selection of recombinant plasmids based on the insertional inactivation of LacZalpha gene cannot differentiate recombinant clones in some cases. Several fragments of exon 11 of human brca1 gene were cloned in LacZalpha-containing plasmids so that frameshift appeared at the 5(')-end of the fragments tested but these fragments were in frame with(More)
Saccharomyces cerevisiae RNA polymerase II general transcription complex proteins (RNA polymerase II, TBP, TFIIB, TFIIF, TFIIE, and TFIIH) were tested for interaction with oligoribonucleotides. Electrophoretic mobility shift assays showed that 32P-labeled CU-rich oligonucleotide p-17 (5′-ACUCUCUUCCG-CAUCGC- 3′) interacted with the proteins to yield three(More)
Increased TATA-binding activity of proteins in nuclear extracts from murine hepatocarcinoma HA-1 and murine Lewis lung adenocarcinoma was demonstrated. The dependence of the amount of formed complexes on protein concentration, displacement of labeled 32P-TATA-containing oligonucleotide by its unlabeled analog, and weak interaction with an oligonucleotide(More)
TATA-binding protein (TBP) is a subunit of basal transcription factor TFIID that recognizes and binds to the TATA-box on TATA-containing promoters of class II genes, and starts assembling RNA polymerase II basal transcription complex. It is shown in many works that the sequence of TATA-box with its flanking regions affects the level of basal and activated(More)
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