Marina G Tarutina

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A method for transformation of the methylotrophic yeast Pichia methanolica (formerly P. pinus MH4) was developed. Mutants leu1 were shown to be transformed with different efficiency using 2.2-10.7-kb linear and circular DNA molecules containing the LEU2 gene of the yeast Saccharomyces cerevisiae, which complemented the leu1 mutation of the recipient.(More)
Two types of genes were found in the study of transformation in yeast Pichia methanolica: transforming (Trg) and nontransforming (Ntg) genes. Transforming genes (P-ADE7,4 and S-LEU2), as linear DNA molecules, can transform competent cells with high efficiency inversely proportional to the molecule size. Nontransforming genes (P-ADE5 and H-LEU2) transform P.(More)
652 spontaneous and 6-N-hydroxylaminopurine and propiolactone-induced mutants were obtained in yeast. 598 of them were LYS2 mutants. Detailed genetic analysis of the mutants was performed, including analysis of growth pattern on lysineless medium, suppressibility by nonsense suppressors of three types and localization on the recombination map of the LYS2(More)
A pNS-cat72 vector was constructed based on the replicative vector pNS2 and reporter gene cat of Tn9 transposone, which encodes chloramphenicol acetyltransferase, in order to clone various gene promoters and to assess their chloramphenicol resistance in Corynebacterium glutamicum strains. The strength of promoters of various genes in the Corynebacterium(More)
In the study of transforming DNA molecules in cells of the methylotrophic yeast Pichia methano-lica, it was found that the efficiency of transformation and the possibility of autonomous replication of hybrid plasmids and miniplasmids consisting of only transforming gene (Trg) sequences do not depend on the presence of ARS sequences with the canonical(More)
Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content.
The collection of overlapping lys2 deletions (five in the chromosomal and seven in the plasmid LYS2 gene) is constructed in this work. The deletions overlap the whole coding region of the gene and provide the system for intragenic recombinational mapping of lys2 mutations in one of 14 controlled regions. A portion of these regions can be correlated with the(More)
By comparing published and experimental data on spontaneous mutability of early genes controlling biosynthesis of purine nucleotides (BPN) in different yeast species in the system “from red to white,” it was shown that the PUR4 gene encoding 5"-phosphoribosylformyl glycinamidine synthetase (FGAM-synthetase) (EC 6.3.5.3) is the most mutable gene in(More)
We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in(More)
Two types of genes were found in the study of transformation in yeastPichia methanolica: transforming (Trg) and nontransforming (Ntg) genes. Transforming genes (P-ADE7,4and S-LEU2), as linear DNA molecules, can transform competent cells with high efficiency inversely proportional to the molecule size. Nontransforming genes (P-ADE5 and H-LEU2) transform P.(More)