Marina Fernandez

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The genotoxicity and/or toxicity of benzo(a)pyrene (BaP) were evaluated under different lighting conditions in larvae and embryos of the newt Pleurodeles waltl. Visible light alone (1,220 lx; 2.1 mW/cm2), UVA alone (250 microW/cm2), or BaP alone (500, 50, 25, 12.5 ppb) had no toxic effects on the larvae. Conversely, toxic effects were observed in animals(More)
We evaluated the influence of near-ultraviolet light (UVA) on the cytotoxicity and genotoxicity of 7 polycyclic aromatic hydrocarbons (PAH) in larvae of the amphibian Pleurodeles waltl. Benz[a]anthracene (BA), 7,12-benz[a]anthraquinone (BAQ) and anthracene (Ac) proved to be lethal at low doses (some ppb), and the following order of genotoxicity was(More)
The micronucleus test using erythrocytes of Pleurodeles waltl larvae (Amphibian, Salamandridae) was used to detect the genotoxic activity of various substances. Most of them were known (or suspected) to be mutagenic or carcinogenic in mammals. Larvae were reared in water containing either a test chemical (experimental group) or in control water and the(More)
The genotoxic potential of various waste waters has been evaluated in a micronucleus test using amphibian larvae. Genotoxicity was detected after dilution, in waste water from tanneries and from various petrochemical industries. Further studies have shown that sample treatment used for in vitro testing may affect the genotoxic response. Sterilization by(More)
A micronucleus test was developed using larvae from two urodele amphibians (Pleurodeles waltl and Ambystoma mexicanum) and an anuran (Xenopus laevis). The methods for maintenance of adults, egg laying, and rearing the larvae are described, and the conditions required for optimal response are given for each of these species. The tests are carried out during(More)
BACKGROUND We have demonstrated that the administration of delta-iodolactone (i.e., 5-iodo-delta lactone) of arachidonic acid (IL-delta), a mediator in thyroid autoregulation, prevents goiter induction by methylmercaptoimidazol (MMI) in rats. Other studies have shown that transforming growth factor beta-1 (TGF-beta1) mimics some of the actions of excess(More)
We have previously described a micronucleus test using erythrocytes from larvae of the urodele amphibians Pleurodeles waltl (pleurodele) and Ambystoma mexicanum (axolotl). The test is based on a comparison of the levels of micronucleated erythrocytes in blood smears from larvae reared in water containing a clastogen, with the levels from larvae reared in(More)
The clastogenic effect of benzo[a]pyrene (B[a]P) was observed in newt larvae by the formation of micronuclei in circulating red blood cells (RBCs). The level of micronucleated RBCs depended on the concentration of B[a]P (0.025, 0.05 and 0.1 p.p.m.) in the water in which the animals were reared. The synthetic antioxidant, 2(3)-tert-butyl(4) hydroxyanisole(More)
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