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Cold stress responses in plants are highly sophisticated events that alter the biochemical composition of cells for protection from damage caused by low temperatures. In addition, cold stress has a profound impact on plant morphologies, causing growth repression and reduced yields. Complex signalling cascades are utilised to induce changes in… (More)
Enolases are key glycolytic enzymes that are highly conserved in prokaryotic and eukaryotic organisms, and are among the most abundant cytosolic proteins. In this study we provide evidence that activity of the enolase ENO2 is essential for the growth and development of plants. We show that Arabidopsis plants with compromised ENO2 function, which were… (More)
Brassinosteroids (BRs) are steroid hormones that are essential for plant growth. Responses to these hormones are mediated by transcription factors of the bri1-EMS suppressor 1/brassinazole resistant 1 subfamily, and BRs activate these factors by impairing their inhibitory phosphorylation by GSK3/shaggy-like kinases. Here we show that BRs induce nuclear… (More)
Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive to freezing before and after cold… (More)
Differences in milk productivity between groups of Black Pied first-calf heifers depending on housing method are determined. Seasonal changes in the indices of immunoglobulin classes G and M are established. The characteristics of daughters of bulls of different origin with respect to the investigated immunoglobulin classes are revealed.
The insulin/insulin-like growth factor signaling pathway is involved in the regulation of the synthesis of insect gonadotropic hormones, juvenile (JH) and 20-hydroxyecdysone (20E). We carried out the immunohistochemical analysis of the insulin receptor (InR) expression in the corpus allatum (the JH-producing gland) and in the ovarian follicular cells (a… (More)
A difference has been established in the live weight of calves during growth from 15 days to 3 months (the growth rate of calves, determined by the relative gain index, on average across the groups was 29.0 and 35.0%) and in the content of immunoglogulins of G and M classes between progenies of two bulls originating from Denmark and Canada. The IgM content… (More)
Research into the immune system status of bull sires is focused on finding the ways to enhance the breeding values of animals and to correct the innate resistance in the organism. The objective of the study was monitoring the immune system status indices and the correlations between them in various bull breeds. It was ascertained that there were more strong… (More)
mutant constructs in transgenic plant lines. CES expression was determined in 2-week-old seedlings of the indicated lines and is shown in comparison to wild-type. GAPC2 was used as an internal control. The error bars are the SD calculated of three independent biological repeats each measured in four technical repeats.