Marijo G. Kent

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The following decisions concerning the banded karyotype of the horse (Equus caballus) were made at the second International conference for Standardization of Domestic Animal Karyotypes, held at Jouy-en Josas, France, 22nd-26th May 1989: (1) numbering of the chromosomes was modified to correspond to an arrangement into only two groups (the non-acrocentrics(More)
Accurate quantification of DNA from cells of several species is possible with flow cytometry. When one species is used as a reference, cytometric readings from two or more different species can be compared to obtain relative percent DNA or DNA indices. Differences in DNA from the male and female of the same species also can be measured. The method allows(More)
The XY sex-reversal syndrome occurs when a phenotypic mare is born that has the karyotype of a stallion. The syndrome is manifested by both genotypic and phenotypic heterogeneity. The sex-reversed genetic condition occurs frequently within certain pedigrees where XY females have been found and can be readily detected by chromosome karyotyping. The(More)
A total of 174 stallions were subjected to a standard fertility examination and classified as fertile, subfertile or sterile. All stallions were phenotypical males involved in breeding programmes with no detectable abnormalities in their reproductive organs. Fertile stallions had no history of any breeding problem. Subfertile stallions were referred with a(More)
H-Y antigen defined by antibody from male-sensitized female mice has been reported in male embryos of mouse, rat, cattle, goat, pig, and sheep. We now describe the use of monoclonal H-Y antibodies in identification of male and female during embryo transfer in cattle. Monoclonal H-Y antibodies were applied with fluorescein-isothiocyanate-conjugated goat(More)
In situ hybridization with a cloned banded krait sex-specific repetitive DNA probe (Bkm) indicates a high concentration of Bkm sequences on the horse Y chromosome in both normal XY males and XY sex-reversed females. Lesser, but still significant, concentrations of Bkm sequences were mapped to horse chromosomes 3, 4, and 30.
An inherited genetic disorder causes XY embryos of the horse to develop as mares. On the basis of our study of 38 such mares, we have identified four grades or classes of XY sex reversal according to this scheme: class I, nearly normal female, of which some are fertile; class II, female with gonadal dysgenesis, normal mullerian development; calss III,(More)
A newly developed enzyme-linked immunosorbent assay (ELISA) was applied for studying H-Y antigen in buffaloes, cattle, horses and humans. A monoclonal H-Y antibody was absorbed with cells from males or females and was then tested against fluid samples known to contain soluble H-Y antigen. In this system, positive absorption manifested itself by a fall in(More)
The XY Sex Reversal Syndrome of the horse is a condition associated with female or intersexual development in genetic males. In our previous study, 38 sex reversed XY mares were classified according to behavior, gross clinical phenotype, gonadal status, and H-Y phenotype. Four classes were described, ranging from potentially fertile female (Class I) to(More)