Marie-Pierre Gramain

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3-D optical fluorescent microscopy becomes nowadays an efficient tool for volumic investigation of living biological samples. Using optical sectioning technique, a stack of 2-D images is obtained. However, due to the nature of the system optical transfer function and non-optimal experimental conditions, acquired raw data usually suffer from some(More)
Except for blind methods, deconvolution of 3-D data sets acquired from a fluorescence microscope requires the knowledge of the point spread function (PSF) of the instrument. Using the XCOSM package, we show first with simulations and then with recorded data that it is possible to recover from an experimental PSF some parameters, which are very difficult or(More)
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