- Full text PDF available (0)
To better understand Escherichia coli O157:H7 on-farm transmission dynamics requires sensitive methods for quantification of a broad range of concentrations of target organisms. For this purpose, a multiplex real time PCR (qPCR) assay was developed for quantification of O157 E. coli from 1g fecal samples of cattle and other animal species, targeting the… (More)
A study is presented of various buffers utilized in the preparation of human lymphocytes for scanning electron microscopy. Of nineteen different buffers tested, Hanks' balanced salt solution +0.04 mol sucrose appeared most adequate for satisfactory preservation of lymphocyte surface architecture.
The fine structure os Strongylocentrotus purpuratis testes has been examined. No obviously important differences appear to exist between the description reported here and the published fine structure of testes obtained from other sources. The most useful fixative was found to be a mixture of glutaraldehyde-paraformaldehyde.
Five protocols were compared to determine the combined effects of different sample weights and culture methods for the recovery of Salmonella from 310 pig cecal samples taken in abattoirs as part of the Canadian Integrated Program for Anti-microbial Resistance Surveillance. Sample weights evaluated were 1 and 10 g. Culture methods used with each sample… (More)