Mariano Villaplana

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Growth hormone (GH) and prolactin (PRL) immunoreactivities in the adenohypophysis of Sparus aurata specimens from newly hatched until 48-months-old were detected using the peroxidase-antiperoxidase method. GH cells and PRL cells, and cells that were immunoreactive to both GH and PRL antisera, called mammosomatotropes (MS cells), were found. This is the(More)
Follicle-stimulating hormone-like gonadotropin (FSH), luteinizing hormone-like gonadotropin (LH), and thyrotropin (TSH) cells were detected in adult and developing pituitary gland of gilthead seabream. Antisera obtained against the alpha- and beta-subunits of FSH (anti-My FSH) and the beta-subunit of LH (anti-My LHbeta), respectively, of the teleost(More)
 This is the first report on the identification of somatolactin (SL) cells during the early developmental stages of the teleost fish Sparus aurata. The SL cells were followed from newly hatched until 46 months. SL cells were immunocytochemically identified at light microscopical level with anti-cod SL in the developing pituitary using the(More)
The cell organization of the pituitary gland and the relationship between neurohypophysis and adenohypophysis in the early developmental stages of the gilthead sea bream, Sparus aurata, were studied by electron microscopy. In newly hatched larvae, the pituitary gland was embedded in the ventral floor of the diencephalon and separated from the hypothalamus(More)
In the pituitary of gilthead sea bream, Sparus aurata, melanotropic (MSH) and adrenocorticotropic (ACTH) cells were identified at the light and electron microscopic levels using rabbit anti-synthetic alphaMSH (MSH) and anti-human ACTH (1-24) (ACTH) sera. The distribution of these cell types was followed from hatching to 48 months. The techniques used(More)
Growth hormone (GH), prolactin (PRL), and mammosomatotrope (MS) cells of gilthead sea bream, Sparus aurata, a teleost fish, were studied in specimens from hatching to 15 months (adults) using conventional electron microscopy and an immunogold method using anti-tilapia GH sera and anti-chum salmon PRL serum. MS cells, immunoreactive to both anti-GH sera and(More)
For the first time, somatolactin (SL) cells have been ultrastructurally identified and characterized during the ontogeny of gilthead sea bream, Sparus aurata, using specimens ranging in age from hatching to 15 months. The SL cells were identified by an immunogold method using anti-cod SL serum. The SL-immunoreactivity was mostly located on the secretory(More)
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