Marianne Woods

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Hepatic vitellogenin (VTG) mRNA and plasma protein assays were developed for the Murray rainbowfish (Melanotaenia fluviatilis), a native freshwater fish species in Australia. Adult male Murray rainbowfish were exposed to 17β-estradiol (E2) or 17α-ethynylestradiol (EE2) at 0, 1, 5, 10, 50, or 100 ng/L in a semistatic system for 7 d. Vitellogenin mRNA was(More)
The localisation of estrogen receptors (ERalpha and ERbeta) and vitellogenin (VTG) transcripts were examined in the liver and testis in male rainbowfish exposed to 17beta-estradiol (E2; 0, 50 and 500 ng/L) via the water for up to 7 days. The ER transcripts were localised within the perinuclear region of the hepatocytes and were up-regulated with E2(More)
Estrogens are steroidal hormones that control many physiological processes in both female and male vertebrates. Like other vertebrates, fish have two distinct estrogen receptors (Esr) subtypes, Esr1 and Esr2a that have been isolated in a number of species, as well as a third subtype, Esr2b. The mRNA encoding the Esr1 was isolated from the female liver of an(More)
The contamination of major continental river systems by endocrine-active chemicals (EACs) derived from the discharge of wastewater treatment plant (WWTP) effluents can affect human and ecosystem health. As part of a long-term effort to develop a native fish model organism for assessment of endocrine disruption in Australia's largest watershed, the(More)
Double strand breaks (DBSs) promote different repair pathways involving DNA end joining or 5 homologous recombination, yet their relative contributions, interplay and regulatory interactions remain to be fully elucidated. These mechanisms give rise to different mutational processes and their propensity for activation directly affects genomic instability(More)
Double strand breaks (DBSs) promote multiple repair pathways and can give rise to different mutagenic processes. The propensity for activation directly affects genomic instability, with implications across health and evolution. However, the relative contribution of these mechanisms, their interplay and regulatory interactions remain to be fully elucidated.(More)
Automated gas-phase protein sequencing has been used to characterize variable regions of antibody heavy and light chains separated by SDS-polyacrylamide gel electrophoresis (PAGE) and electroblotted onto Immobilon polyvinylidene difluoride membranes ('blot-sequencing'). Starting from 100 micrograms of antibody, 20 or more residues of N-terminal VH and VL(More)
Double strand breaks (DBSs) promote multiple repair pathways and can give rise to different mutagenic processes. The propensity for activation directly affects genomic instability, with implications across health and evolution. However, the relative contribution of these mechanisms, their interplay and regulatory interactions remain to be fully elucidated.(More)
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