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Ovarian tissue of prenatal, newborn, and 5-day-old rats does not specifically bind 125I-labelled HCG. Specific binding of HCG was first observed in ovaries of 10-day-old animals and binding increased with age. These results indicate that, contrary to rat testis, the HCG receptor in the rat ovary is not present during foetal and early postnatal development.(More)
Assisted conception is a useful methodology for detecting disturbances in clinical outcome, meiotic maturation, and genetic integrity of human gametes. Germinal cells are vulnerable to genetic damage from smoking, but can repair damage during meiosis. In ejaculated spermatozoa, repair capacity declines drastically. Smoking alters the meiotic spindle of(More)
OBJECTIVE To evaluate two different assays of human sperm DNA integrity, DNA denaturation (DD) and DNA fragmentation (DF), and to correlate these with standard semen parameters. DESIGN Prospective, observational study. SETTING University infertility clinic. PATIENT(S) Forty consecutive semen samples from 33 nonazoospermic men presenting for(More)
The binding capacity for H-Y antigen was studied in various rat tissues of both sexes. In nongonadal tissues (liver, kidney, brain, epidermis) binding could not be demonstrated. In contrast, the gonads are able to bind exogenously supplied H-Y antigen. In the ovary, the binding capacity remains unchanged in newborn and adult animals, while in the testis,(More)
Various cell types of the rat testis during pubescence, including germ, Sertoli, and Leydig cells, were partially enriched. The fractions were tested for the presence, binding, and secretion of H-Y antigen. The main results are: Immature germ cells are H-Y antigen-negative until the late diploid stages, and late primary spermatocytes or spermatids become(More)
OBJECTIVES To assess the rate of chromosome aneuploidy (e.g., extra or missing chromosomes) in oocytes remaining unfertilized in our in vitro fertilization (IVF) program. To determine whether two parameters of the IVF technique, advanced maternal age and hormonal follicle stimulation, affect this rate. DESIGN Data on oocyte retrieval, fertilization, and(More)
Testes of either newborn rats or newly hatched chickens, dissociated into single cell suspensions, reorganize in vitro into their histotypic structures. In birds, the heterogametic female sex is H-Y antigen positive, and not the male as in mammals. Cocultivation of rat and chicken testicular cells results in the reorganization of an ovotestis. A similar(More)
OBJECTIVE To assess cadmium, a heavy metal in cigarette tobacco, in follicular fluid (FF) of women in IVF-ET, who smoke. DESIGN Controlled clinical study. SETTING Infertile patients in a hospital IVF-ET program. PATIENTS Fifty-one women selected in groups according to smoking habits: nonsmokers (n = 10), passive smokers (n = 17), light smokers (< 15(More)
From 67 diploid male Chinese hamster cells in vitro, an idiogram was constructed. The chromosomes can be classified into 4 natural groups: group I, 2 pairs of large submetacentrics (1 and 2); group II, 3 pairs of medium-sized submetacentrics (the sex pair, 4 and 5); group III, 3 pairs of medium-sized subtelocentrics (6,7, and 8); group IV, 3 pairs of small(More)
OBJECTIVE To assess cotinine, a metabolite of nicotine, in follicular fluids (FF) of women who smoke either actively or passively or not all. DESIGN Controlled clinical study. SETTING Infertile patients in a hospital IVF-ET program. PATIENTS One hundred eleven women classified by smoking habits: active smokers (n = 44), passive smokers (n = 17), or(More)