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Assisted conception is a useful methodology for detecting disturbances in clinical outcome, meiotic maturation, and genetic integrity of human gametes. Germinal cells are vulnerable to genetic damage from smoking, but can repair damage during meiosis. In ejaculated spermatozoa, repair capacity declines drastically. Smoking alters the meiotic spindle of(More)
OBJECTIVE To evaluate two different assays of human sperm DNA integrity, DNA denaturation (DD) and DNA fragmentation (DF), and to correlate these with standard semen parameters. DESIGN Prospective, observational study. SETTING University infertility clinic. PATIENT(S) Forty consecutive semen samples from 33 nonazoospermic men presenting for(More)
OBJECTIVE To assess cadmium, a heavy metal in cigarette tobacco, in follicular fluid (FF) of women in IVF-ET, who smoke. DESIGN Controlled clinical study. SETTING Infertile patients in a hospital IVF-ET program. PATIENTS Fifty-one women selected in groups according to smoking habits: nonsmokers (n = 10), passive smokers (n = 17), light smokers (< 15(More)
OBJECTIVE To assess cotinine, a metabolite of nicotine, in follicular fluids (FF) of women who smoke either actively or passively or not all. DESIGN Controlled clinical study. SETTING Infertile patients in a hospital IVF-ET program. PATIENTS One hundred eleven women classified by smoking habits: active smokers (n = 44), passive smokers (n = 17), or(More)
We investigated whether nicotine exposure in vitro of mouse oocytes affects spindle and chromosome function during meiotic maturation (M-I and M-II). Oocytes in germinal vesicle (GV) stage were cultured in nicotine for 8 h or for 16 h, to assess effects in M-I and in metaphase II (M-II). The latter culture setting used the three protocols: 8 h nicotine then(More)
Benzo[a]-pyrene (B[a]P) is a potent mutagen and carcinogen present in cigarettes. We report here on immunodetection and quantification of B[a]P-DNA adducts in granulosa-lutein cells of patients undergoing in-vitro fertilization (IVF) and embryo transfer, who were exposed to cigarette smoke. Follicular fluids (FF) and granulosa-lutein cells were obtained(More)
OBJECTIVE To determine the effects of chilling to 0 degrees C on the meiotic spindle of human metaphase II oocytes, as observed by optical sectioning microscopy. DESIGN Laboratory study. SETTING Academic research laboratory in a medical school. PATIENT(S) Seventy-two women undergoing infertility treatment donated a total of 108 oocytes. (More)
There is a strong association between cigarette smoking and reduced fecundity, reduced fertility, and early mean age of menopause, suggesting that smoking may impair oocyte function and viability. We analysed the effect of smoking on meiotic maturation of oocytes. A total of 156 women undergoing in-vitro fertilization therapy, classified as non-smokers (n =(More)
PURPOSE Our purpose was to determine whether there is variation in levels of follicular fluid (FF) cotinine between the two ovaries of women undergoing IVF-ET who are exposed to cigarette smoke. METHODS In 61 women, there were two to four determinations of FF continine levels for each of two follicles, one from each ovary. For each woman a t test for(More)