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In the first experiment, mature female Wistar rats, displaying a regular 4-day oestrous cycle, were killed in succession every 2 or 3 h on the day of pro-oestrus and oestrus until the time of ovulation. In the second experiment, immature female Wistar rats (aged 24 days) were injected s.c. with 30 IU pregnant mare serum gonadotrophin (PMSG) and 56 h later… (More)
Sequential changes in androgen receptor (AR) distribution were investigated in rat ovarian follicles during their physiological development. Mature female Wistar rats, exhibiting a regular 4-day oestrous cycle, were killed in succession on the day of oestrus, metoestrus, dioestrus, and pro-oestrus. Excised ovaries were submitted to immunohistochemical… (More)
The process of apoptosis in the postovulatory cumulus granulosa cells was investigated in pregnant rats. Mature female Wistar rats, exhibiting a regular 4-day oestrous cycle, were placed with males on the day of pro-oestrus. The following day, on which spermatozoa were found in vaginal smears, was designated day 1 of pregnancy. The animals were killed just… (More)
In the rat, at the beginning of pregnancy a cohort of antral follicles develops until the preovulatory stage. However, these follicles, differentiating in the hyperprolactinemic milieu, produce only small amount of estradiol, do not ovulate and undergo rapid degeneration. They constitute an interesting physiological model of atresia. In the present study,… (More)
Immunohistochemical localization of androgen receptor (AR) was investigated in the rat oocytes during their development in the primary, secondary and antral follicles. The group of experimental Wistar rats included prepubertal female rats, killed at 30 days of age, and mature female rats killed at estrus or metestrus. Excised ovaries were submitted to… (More)
Distribution of alpha-tubulin was investigated in the cumulus oophorus complexes (COCs) of the rat using anti-alpha-tubulin monoclonal antibody and fluorescence microscopy. Localization of alpha-tubulin was assessed in the compact and fully expanded COCs isolated directly from the preovulatory follicles and in the cryosections containing these structures.… (More)
The levels of oestrogens in isolated follicles of rats were low until day 3 of pregnancy then elevated during the next three days and were again low until day 21. On that day, a sudden rise took place which continued on day 22. Follicular androgens followed the pattern of oestrogens. Progestagen amounts were virtually negligible.
In the present study the authors investigated whether androgens could interact with FSH to induce aromatase and androgen receptor expression in porcine granulosa cells. Dissected whole porcine follicles (small, medium, and large) were incubated for 8 hours in M199 medium supplemented with testosterone (10(-7) M), FSH (100 ng/ml) or both those hormones.… (More)
The amounts of follicular oestrogens increased simultaneously with the growth of follicles to reach a maximum on the day of pro-oestrus at 18:00 h, then decreased rapidly. Progestagens were detectable only in large follicles excised at pro-oestrus. Small follicles at pro-oestrus contained small amounts of oestrogens.
The immunohistochemical localisation of androgen receptor (AR) was investigated in a cohort of ovarian antral follicles developing, and subsequently undergoing atresia, in a hyperprolactinaemic milieu at the beginning of pregnancy in rats. Differentiation of the investigated follicles, observed during the first 5 days of pregnancy, was accompanied by a… (More)