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The tripeptide glutathione is a major antioxidant and redox buffer with multiple roles in plant metabolism. Glutathione biosynthesis is restricted to the cytosol and the plastids and the product is distributed to the various organelles by unknown mechanisms. In the present study immunogold cytochemistry based on anti-glutathione antisera and transmission(More)
Compartment specific changes in ascorbate and glutathione contents were studied during drought stress in Arabidopsis thaliana Col-0 and in ascorbate and glutathione deficient mutants vtc2-1 and pad2-1, respectively, over a time period of 10 days. The results of this study revealed a strong decrease of glutathione contents in both mutants (up to 52% in(More)
Wir berichten über den ersten dokumentierten Leptospirose-Ausbruch in Österreich. Im Juli 2010, erkrankten vier Athleten nach Teilnahme am Bergwerksee Langau-Triathlon an serologisch verifizierter Leptospirose. Schwere Regenfälle hatten am Vortag des Sportereignisses Niederschläge von 22 mm erbracht. Der Indexpatient (Patient A) war ein 41-Jähriger, der am(More)
The intracellular effects of GSH (reduced glutathione) and BSO (buthionine sulfoximine) treatment on glutathione content were investigated with immunogold labeling in individual cellular compartments of Cucurbita pepo L. seedlings. Generally, GSH treatment led to increased levels of glutathione in roots and leaves (up to 3.5-fold in nuclei), whereas BSO(More)
This study describes the subcellular distribution of glutathione in roots and leaves of different plant species (Arabidopsis, Cucurbita, and Nicotiana). Glutathione is an important antioxidant and redox buffer which is involved in many metabolic processes including plant defense. Thus information on the subcellular distribution in these model plants(More)
The extent of liver and kidney damage in 10 young steers, infected with a stabilate of Theileria annulata, was estimated by the determination of enzymes (GOT, GPT, SDH, ALD), serum levels of bilirubin and urea. At the same time the effectiveness of some liver protecting medical agents was tested. The following results were obtained: Change in the activity(More)
Electronmicroscopic immunogold cytochemistry was used to investigate the cellular and subcellular distribution of glutathione in root and leaf cells of Styrian pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) plants. Gold particles bound to glutathione were found in various cell structures. Statistical evaluation of the gold particle density was(More)
 The present research demonstrates severe ultrastructural changes induced by zucchini yellow mosaic virus (ZYMV) within the cells of older and younger leaves of Styrian pumpkin plants (Cucurbita pepo L. subsp. pepo var. styriaca GREB.). Cylindrical inclusions (pinwheels), proliferated endoplasmatic reticulum and filamentous viral particles were found(More)
BACKGROUND AND AIMS In the present study, the differences between glandular and non-glandular trichomes, the secretory process and the method of secretion were studied. Previous studies on leaves of Styrian oil pumpkin (Cucurbia pepo var. styriaca) plants have shown that four morphologically and ontogenetically independent glandular and non-glandular(More)
Plants cope with cadmium (Cd) stress by complexation with phytochelatins (Pc), metallothioneins and glutathione and sequestration within vacuoles. Especially glutathione was found to play a major role in Cd detoxification as Cd shows a high binding affinity towards thiols and as glutathione is a precursor for Pc synthesis. In the present study, we have used(More)