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The experimental data of the past decade concerning the metabolic peculiarities of aerobic methylobacteria and the prospects for their use in different fields of modern biotechnology, including genetic engineering techniques, have been summarized.
Aerobic methylotrophic bacteria able to grow with dichloromethane (DCM) as the sole carbon and energy source possess a specific glutathione S-transferase, DCM dehalogenase, which transforms DCM to formaldehyde, used for biomass and energy production, and hydrochloric acid, which is excreted. Evidence is presented for chloride-specific responses for three… (More)
A knockout mutant of Methylobacterium dichloromethanicum DM4 with an inactivated gene of a putative transcription regulator METDI5511 (ΔMETDI5511) has been obtained. The expression of this gene increases many times when the strain is grown on dichloromethane compared to methanol. The mutant had a low growth rate on dichloromethane as compared with the… (More)
Activation of expression of the xoxF gene encoding PQQ-dependent methanol/ethanol dehydrogenase (METDI2492) in dichloromethane (DCM)-grown Methylobacterium dichloromethanicum DM4 was first demonstrated. The sequence of the only XoxF homolog found in the genome of strain DM4 exhibited 50% identity to that of the protein (MxaF) of the large subunit of… (More)