Maria-Honorina C. Lopes

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Objective and design: Determine the sources of nitric oxide (NO) and evaluate its role in the activation of nuclear Factor-kappaB (NF-κB) and activator protein-1 (AP-1) and in the expression of NO synthase II (NOS II), induced by interleukin-1β (IL-1).¶Material or subjects: Primary cultures of bovine articular chondrocytes.¶Treatment: The cells were treated(More)
The pro-inflammatory cytokine interleukin-1β (IL-1) induces articular chondrocytes to produce reactive oxygen species (ROS), including hydrogen peroxide (H2O2), which mediate some IL-1-induced responses. This study aimed at elucidating the role of ROS, particularly H2O2, in mediating IL-1-induced activation of the transcription factor activator protein-1(More)
Several evidences suggest that the pro-inflammatory cytokines IL-1 beta and the radical NO are implicated as effectors molecules in the pancreatic beta-cells dysfunction; an event preceding the pathogenesis of diabetes. IL-1 beta induces the expression of the inducible isoform of NO synthase (iNOS), which use L-arginine as substrate to overproduce NO.(More)
The mechanisms by which glucocorticoids (GC) regulate annexin I (ANXA1) secretion in different cells are still a matter of debate. The aims of this study were to evaluate the ability of dexamethasone (Dex) to induce ANXA1 secretion and to investigate the roles of the intracellular free Ca2+ concentration ([Ca2+]i), and of the GC receptor, on that process.(More)
The Janus kinase (JAK) family of protein tyrosine kinases are activated in response to a wide variety of external stimuli. Here we have investigated whether the janus kinase 2 (JAK2) is involved in the induction of nitric oxide synthase type II (iNOS) expression in a mouse fetal skin dendritic cell line (FSDC). In FSDC the expression of iNOS protein and(More)
Insulin-like growth factor I and II (IGF-l and IGF-ll) have been implicated in the replication of normal thyroid follicular cells in vitro. This study evaluates the distribution and abundance of immunoreactive IGF-l by histochemical analysis in human thyroid tissue with different histopathologic characteristics. We used two types of highly specific and(More)
Ca2+ channel blockers belonging to three distinct chemical groups (dihydropyridines, phenylalkylamines and diphenylalkylamines) differentially inhibit the (Ca2+ + Mg2+)-ATPase activity of synaptic plasma membranes (Santos et al., J. Neurochem. 52, S49D, 1989). We now report that (-)-desmethoxyverapamil and flunarizine are the most potent inhibitors of the(More)
The multiplication of Bhanja virus in CV-1 cells was studied both by staining the viral antigens with the FA technique and measuring the light intensity emitted by the fluorescent cells with a photomultiplier and by the simultaneous titration of intracellular and extracellular virus. The fluorescence appeared at 3 hrs post adsorption in the form of very(More)
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