Maria Elisabete Cunha Dias Real Oliveira

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This study describes a novel liposomal formulation for siRNA delivery, based on the mixture of the neutral lipid monoolein (MO) and cationic lipids of the dioctadecyldimethylammonium (DODA) family. The cationic lipids dioctadecyldimethylammonium bromide (DODAB) and chloride (DODAC) were compared in order to identify which one will most efficiently induce(More)
We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High adsorption efficiency (91.0 ± 9.0%) is attained with high lipid(More)
PURPOSE The aim of this study was to, firstly, investigate whether silicone-hydrogel contact lenses (CL) are more or less susceptible to bacterial adhesion than conventional ones and, secondly, assess the influence of lens wear in the extent of bacterial adhesion. Four silicone-hydrogel CL (galyfilcon A, balafilcon A, lotrafilcon A, and lotrafilcon B) and(More)
A recently described non-viral gene delivery system [dioctadecyldimethylammonium bromide (DODAB)/monoolein (MO)] has been studied in detail to improve knowledge on the interactions between lamellar (DODAB) and non-lamellar-forming (MO) lipids, as a means to enhance their final cell transfection efficiency. Indeed, the morphology, fluidity, and size of these(More)
PURPOSE This study was designed to qualitatively and quantitatively characterize the surface morphology of four unworn conventional hydrogel contact lenses (omafilcon, hioxifilcon, nelfilcon A, and ocufilcon B) and two silicone-hydrogel contact lenses (senofilcon A and comfilcon A) without surface treatment. METHODS Surface roughness was assessed using(More)
PURPOSE The main goal was to study the biochemical composition of the tear film in two different times of the day. METHODS Tear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collected twice in the day, one early in the(More)
PURPOSE The purpose of this work was to evaluate the ability of four silicone-hydrogel contact lenses (galyfilcon A, balafilcon A, lotrafilcon A and lotrafilcon B) to retain their equilibrium water content before and after wear, through measurements of refractive index and compare with that of a conventional disposable hydrogel contact lens (etafilcon A).(More)
PURPOSE The aim of this work was to study the central and peripheral thickness of several contact lenses (CL) with different powers and analyze how thickness variation affects CL oxygen transmissibility. METHODS Four daily disposable and five monthly or biweekly CL were studied. The powers of each CL were: the maximum negative power of each brand; -6.00(More)
PURPOSE The aim of this study was to evaluate the influence of lens material and lens wear on the removal capability of Staphylococcus epidermidis. Assessment of viability of remaining adhered bacteria was another goal of this work. Four silicone hydrogel materials (galyfilcon A, balafilcon A, lotrafilcon A, lotrafilcon B) and one conventional hydrogel(More)
PURPOSE To evaluate the influence of wear of silicone-hydrogel contact lenses on lens ultraviolet and visible spectrum transmittance by studying several contact lenses before and after wear. METHOD To investigate the ability of contact lenses to maintain their transmittance characteristics, we measured 104 different contact lenses in the ultraviolet (UV)(More)