Maria Beatriz N. Souza

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We report the construction and use of a new system for the direct screening of recombinant clones after transformation. The system uses a Bacillus subtilis-Escherichia coli shuttle vector that carries the B. subtilis structural gene for α-amylase. Insertion of foreign DNA into this gene results in a loss of amylolytic activity in the host cells that can be(More)
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