Maria-Anna Rapsomaniki

Learn More
Once-per-cell cycle replication is regulated through the assembly onto chromatin of multisubunit protein complexes that license DNA for a further round of replication. Licensing consists of the loading of the hexameric MCM2-7 complex onto chromatin during G1 phase and is dependent on the licensing factor Cdt1. In vitro experiments have suggested a two-step(More)
Background: MCM2-7 loading onto chromatin licenses origins for replication. Results: MCMs exhibit transient interactions with chromatin in late mitosis, stable binding in G1, and increased loading in late G1. Conclusion: Multi-level regulation of MCM2-7 loading to chromatin occurs during mitosis and preceding the G1/S phase transition. Significance: The(More)
  • 1