Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
- I. Matic, J. Schimmel, A. Vertegaal
- Biology, ChemistryMolecules and Cells
- 27 August 2010
Stalling of spliceosome assembly at distinct stages by small-molecule inhibitors of protein acetylation and deacetylation.
- A. Kuhn, Maria A van Santen, A. Schwienhorst, H. Urlaub, R. Lührmann
- Biology, ChemistryRNA: A publication of the RNA Society
- 1 November 2008
Mass-spectrometric analysis of affinity-purified stalled spliceosomes indicated that the intermediates differ in protein composition both from each other and from previously characterized native A and B splicing complexes, which suggests that the stalled complexes represent hitherto unobserved intermediates of splicingosome assembly.
Synthesis and Characterization of Pseudocantharidins, Novel Phosphatase Modulators That Promote the Inclusion of Exon 7 into the SMN (Survival of Motoneuron) pre-mRNA*
- Zhaiyi Zhang, O. Kelemen, S. Stamm
- BiologyJournal of Biological Chemistry
- 10 January 2011
The data show that subtle changes in similar compounds can turn a phosphatase inhibitor into an activator, and chemically related compounds influence alternative splicing by distinct mechanisms.
The intronic splicing code: multiple factors involved in ATM pseudoexon definition
- A. Dhir, E. Buratti, Maria A van Santen, R. Lührmann, F. Baralle
- BiologyEMBO Journal
- 17 February 2010
A complex combinatorial interplay of RNA structure and trans‐acting factors in determining the splicing outcome is suggested and contribute to understanding the intronic splicing code for the ATM pseudoexon.
ATPγS stalls splicing after B complex formation but prior to spliceosome activation
- D. E. Agafonov, Maria A van Santen, R. Lührmann
- Biology, ChemistryRNA: A publication of the RNA Society
- 1 September 2016
Affinity purification of ATPγS-stalled B complexes and subsequent characterization of their abundant protein components revealed that BATPγS complexes are compositionally more homogeneous than B complexes previously isolated in the presence of ATP, indicating that these proteins are recruited after assembly of the precatalytic spliceosome.