Margot Kaelbling

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Inactivation of the protein product of the wild-type tumour suppressor gene p53 through complexing of the protein with the E6 oncoprotein of human papillomaviruses (HPV) in HPV-infected cells is thought to be important in the aetiology of cervical carcinoma. Mutations of p53 have also been reported in HPV-negative carcinomas, and we now demonstrate loss of(More)
Electron microscopy of synaptonemal complexes in heterokaryotypic Gallus domesticus cockerels revealed that chromosome rearrangements that had been classified by light microscopy as single breaks were in fact reciprocal translocations. A microchromosome provided the centromere for the acentric long arm of chromosome 1 in a rearrangement that had been(More)
Synaptonemal complexes (SC's) were compared to their chromosomes by transmission electron microscopy (TEM) of whole-mount spreads from 42 male chickens. Individual macrochromosomal SC's were first identified in heterokaryotypes and later by their centromere index. For all nine macrochromosomes (eight autosomes and the Z sex chromosome), the relative length(More)
An analysis for cosegregation of chromosomes and tumorigenicity in 52 hybrids of human diploid X D98AH2 human carcinoma-derived cells reveals the consistent presence of four copies of chromosome 11 in all nontumorigenic hybrids (two from each of the parental cells) and a consistent loss of one or two copies of the 11 in all tumor cells derived from(More)
Retrovirus receptors remain a largely unexplored group of proteins. Of the receptors which allow infection of human and murine cells by various retroviruses, only three have been identified at the molecular level. These receptors include CD4 for human immunodeficiency virus, Rec-1 for murine ecotropic virus, and GLVR1 for gibbon ape leukemia virus. These(More)
Tumorigenicity assays of 48 intraspecies hybrids between human carcinoma-derived cells of the D98AH2 (HeLa) cell line and normal human diploid cells revealed that most are nontumorigenic in nude mice. Chromosome analysis revealed that these hybrids contained four chromosomes 11, presumably two from each parental cell. Their tumorigenic segregants sometimes,(More)
Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Alu I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small(More)
Studies with cell hybrids of normal diploid cells fused with tumorigenic D98AH2 (D98) cells had implicated human chromosome 11 of a normal cell as carrying tumorigenicity suppressing information. The cervical carcinoma-derived D98 (HeLa) cells contain two copies of chromosome 11. In this study, analysis of restriction fragment length polymorphism of DNA(More)
Electron microscopy of synaptonemal complexes in three Gallus domesticus cockerels that were heterokaryotypic for a pericentric inversion in chromosome 2 revealed a low incidence of homologous pairing and a high incidence of nonhomologous pairing. The significance of these results are related to the finding that heterokaryotypic parents have fertility rates(More)
The locations of the genes for fibronectin (FN) on chromosomes of human germ line and somatic cells were determined by in situ molecular hybridization with two 3H-labeled DNA probes, one for the region encoding the cell attachment domain of human FN, the other for the 3' noncoding and part of the coding region. Pachytene chromosomes of two males and(More)