Margarida Santos-Reis

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The genetic diversity of Borrelia burgdorferi sensu lato was assessed in individual adult Ixodes ricinus ticks from Europe by direct PCR amplification of spirochetal DNA followed by genospecies-specific hybridization. Analysis of mixed infections in the ticks showed that B. garinii and B. valaisiana segregate from B. afzelii. This and previous findings(More)
Mediterranean ecosystems are inherently patchy, challenging habitat-use behavior. Certain mammalian carnivores take advantage of this patchiness by a strategy of habitat complementation/supplementation, which is invariant to the scale of analysis. To test if the same behavior is adopted by the stone marten, we used a combined data set of capture and(More)
We isolated and characterised 13 novel polymorphic tetranucleotide microsatellite loci in the stone marten (Martes foina) from genomic libraries enriched for (AAAG) n and (AGAT) n repeat sequences. Thirty-three individuals were genotyped and the number of alleles per locus recorded varied between two and eight and their observed and expected(More)
This work is an updated revision of the available information on Portuguese ixodid tick species. It includes data on tick biology, ecology, taxonomy and host/pathogen-associations. The current list of Portuguese ixodid ticks comprises twenty species: Dermacentor marginatus (Sulzer, 1776), Dermacentor reticulatus (Fabricius, 1794), Haemaphysalis hispanica(More)
Four species of otters (Mustelidae, Lutrinae) occur in Southeast Asia and are considered to be of conservation concern: Aonyx cinerea (Asian small-clawed otter), Lutra lutra (Eurasian otter), Lutra sumatrana (Hairy-nosed otter), and Lutrogale perspicillata (Smooth-coated otter). Among these, L. sumatrana is endemic to the region, yet little is known about(More)
In Portugal, Ixodes ricinus ticks have been shown to contain DNA of several spirochetes belonging to the Borrelia burgdorferi sensu lato complex, with major differences in the genetic diversity between ecozones. Some isolates have been obtained since 1999, confirming the circulation of pathogenic strains in these ticks. Ixodes ricinus is considered to be a(More)
Genetic species identification of non-invasively collected samples has become an important tool in ecological research, management and conservation and wildlife forensics. This is especially true for carnivores, due to their elusive nature, and is crucial when several ecologically and phylogenetically close species, with similar faeces, hairs, bones and/or(More)
The interaction between hunting interests and legally protected predators is often a contentious conservation problem, requiring detailed understanding of predator responses to game management. This issue was addressed in southern Portugal in a treatment-control natural experiment, whereby the abundances of small game, corvids, birds of prey and carnivores(More)
The role of rodents as Leptospira renal carriers in Terceira island was evaluated (1993–1995) through kidney culture and serology [microscopic aglutination test (MAT)] of 94 mice and rats. Fifty-nine animals were positive (n = 41 by serology + culturing; n = 11 serology; n = 7 culturing), presenting a wide distribution in man-made and natural areas. House(More)