Margaret Ulrich

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OBJECTIVE To characterize the mRNA expression of CXC chemokine IL-8, CC chemokine monocyte chemothractant protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES), and a newly defined DC chemokine DC-CK1 as well as the expression of IL-8 receptor, MCP-1 receptor and RANTES receptor in human monocyte-derived dendritic(More)
Cytokinins influence several fundamental processes of plant growth and development, including cell division and organogenesis. To identify genes involved in cytokinin response, a screen was carried out for mutants of Arabidopsis thaliana (L.) Heynh. that are resistant to elevated levels of exogenous cytokinins. One such mutant was isolated and named cyr1. A(More)
Prostaglandin (PG) E1 or E2 added at 2-1,000 ng/ml to quiescent cultures of Swiss 3T3 cells synergistically enhanced the rate of initiation of DNA replication stimulated by PGF2 alpha alone or with insulin. Neither PGD2 nor PGF1 alpha had any effect with PGF2 alpha. An increase in the rate of entry into S phase also occurred when PGE1 or PGE2 was added 8 or(More)
IgE anti-DNP antibody-secreting hybridomas were obtained by polyethylene glycol fusion of murine myeloma cells with spleen cells from mice, primed with DNP-KLH and challenged with DNP-Nippostrongylus brasiliensis extract. The in vivo and in vitro continuously growing hybridomas are producing high amounts of monoclonal IgE anti-DNP antibodies, which are(More)
The stimulatory effect of epidermal growth factor, alone or with insulin, on the rate of initiation of DNA synthesis in Swiss 3T3 cells can be synergistically enhanced by the addition of either Colcemid or colchicine at 1 microM. However, both Colcemid and colchicine can exert the synergistic effect only when added earlier than 8 hr of the prereplicative(More)
Interferon-γ (IFN-γ) and interleukin-4 (IL-4) are key effector cytokines for the differentiation of T helper type 1 and 2 (Th1 and Th2) cells. Both cytokines induce fate-decisive transcription factors such as GATA3 and TBX21 that antagonize the polarized development of opposite phenotypes by direct regulation of each other's expression along with many other(More)
Epidermal growth factor (EGF) stimulates the initiation of DNA synthesis in Swiss 3T3 cells after a constant prereplicative period of 14-15 hours. The final rate of initiation follows apparent first-order kinetics and can thus be quantified by a rate constant k. The value of k can be changed by later additions during the prereplicative period: When cells(More)
The antibody response was analysed in rabbits oro-gastrically infected (i) with virulent-(plasmid-carrying) Yersinia enterocolitica 0:3 (v-rabbits) and (ii) with the avirulent (plasmid-cured) derivative (av-rabbits). In an immunoblot assay with whole cell lysate proteins from the infecting virulent Yersinia strain, a significant IgG response was evident in(More)