Margaret J. Doherty

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At postconfluence, cultured bovine pericytes isolated from retinal capillaries form three-dimensional nodule-like structures that mineralize. Using a combination of Northern and Southern blotting, in situ hybridization, and immunofluorescence we have demonstrated that this process is associated with the stage-specific expression of markers of primitive(More)
Pericytes, an integral part of the microvasculature, are involved in a number of different processes, including angiogenesis. Many of the early studies on these cells are descriptive and concentrate on the location of pericytes in vivo, surrounding the endothelial cells in the microvessels. These studies led to the proposals that pericytes have a function(More)
BACKGROUND Previous studies have shown that pericytes can differentiate into osteoblasts and form bone. This study investigated whether pericytes can also differentiate into chondrocytes and adipocytes. METHODS AND RESULTS Reverse transcription-polymerase chain reaction demonstrated that pericytes express mRNA for the chondrocyte markers Sox9, aggrecan,(More)
PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified(More)
When the TCR binds Ag it is phosphorylated, internalized, and degraded. We wished to examine whether this process was accompanied by a specific concomitant increase in TCR mRNA levels. To do this, PBMC and a T cell clone were cultured with a series of superantigens and an alloantigen. Only T cells specifically responding to an antigenic stimulus had(More)
A conference for social workers was held at the Manhattan State Hospital in connection with the Quarterly Conference of the Department on September 15 and 16, 1931. Dr. Parsons presided at the morning session at which there was an unusually large attendance. The guests were welcomed by Dr. Jaeob Osldag of the Board of Visitors of the Manhattan State(More)
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