Margaret E. Elmes

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The distribution of Paneth cells in the rat small intestine was found to be approximately one per crypt in the duodenum, two to three per crypt in the jejunum and four to six per crypt in the ileum. The ileum has more Paneth cells containing dithizone-reactive zinc than the duodenum and jejunum. Zinc deficiency had no significant effect on the total Paneth(More)
The aim of this study was to contribute to the understanding of the pathogenesis of copper-induced damage and subsequent recovery and tolerance to copper in the copper-loaded rat liver. Male Wistar rats were allocated randomly into groups of four, fed a pelleted diet containing 1500 mg/g copper, and killed at 1, 5, 6, 10, and 15 weeks. Two additional groups(More)
The number of apoptotic bodies found in the small intestine of control non-fasted rats was low. Apoptotic bodies were found in the crypt region of zinc-deficient rats and were present in very large numbers in half the animals examined. Increased numbers of apoptotic bodies were found in the crypt and fundic regions of fasted rats. The failure of cellular(More)
The Paneth cell population in surgically resected human jejunum and ileum was estimated using image analysis of the granule area in patients who had not received antibiotics. The mucosa was cultured aerobically and anaerobically. In the jejunum 22 samples were sterile and five yielded bacteria; in the ileum four were sterile and three were non-sterile. The(More)
The relationship between immunohistochemically demonstrable hepatic metallothionein (MT) and age was studied in histologically normal livers from 12 fetuses (13-28 weeks' gestation) and 19 children (birth-4 years). In addition, the relationships between copper (rubeanic acid-positive), copper-associated protein (orcein-positive), lipofuscin (periodic acid(More)
A sensitive immunohistochemical technique was used to demonstrate zinc metallothionein in rat and human liver and ileum. In the liver, immunoreactivity was observed within the hepatocyte nucleus and cytoplasm, in sinusoids, canaliculi and blood vessels. In the ileum, immunoreactivity was present in the enterocyte nucleus and cytoplasm, and in the lamina(More)
Antibody to rat liver metallothionein prepared by the method of Brady and Kafka (1979) was used to localise immunoreactive metallothionein using a sensitive DNP hapten sandwich technique applied to formalin fixed wax embedded tissues. Rat tissues examined were liver, kidney and small intestine, taken from normal animals, from animals fasted after receiving(More)
Histochemical methods do not always show a good correlation with analytical measurement of copper content and consequently immunoreactive staining techniques for metallothionein (MT) have recently been employed for the differential diagnosis of copper-associated diseases. This study compares histochemical with immunocytochemical methods for the assessment(More)