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The present study focuses on application of quantitative methods measuring differences between particular morphological types of microglial cells as well as between their proliferating and non-proliferating examples. On the basis of subjective classification, microglial cells of three morphological types (ramified, hypertrophied and bushy) were selected(More)
The inflammation is a response of the organism to damaging factors and leads to the limitation of the tissue destruction. During the inflammatory process, there is stimulation of the immune system as well as other tissue cells. However, sometimes this reaction is excessive and can bring to the sepsis and development of multiorgan insufficiency. Phenomena(More)
Following a mechanical lesion of the left cerebral hemisphere, newborn male rats received a single injection of recombinant rat interferon gamma (IFN gamma) into the lesion cavity at doses of 5, 50 and 500 U. One or two days after the injury the rats were injected with 3H-thymidine. Brain sections were immunostained for glial fibrillary acidic protein(More)
Pregnant Wistar rats were exposed to a single 1.0 Gy dose of gamma-irradiation on gestational day 13, 15, 17 or 19. Thirty-day-old male offspring received a mechanical lesion in the left cerebral hemisphere. One, 2 or 4 days after the injury the rats were injected with [3H]thymidine and sacrificed 4 h after the injection. Thereafter, brain sections were(More)
  • Marek Ziaja
  • 2013
Every year, more cases of sepsis appear in intensive care units. The most frequent complication of sepsis is septic encephalopathy (SE), which is also the essential determinant of mortality. Despite many years of research, it still is not known at which stage of sepsis the first signs of SE appear; however, it is considered the most frequent form of(More)
Changes in the number and distribution of microglial cells proliferating in response to unilateral injury of the cerebral hemisphere were investigated in 30-day-old rats. Twelve hours or 1, 2, 4, or 8 days following the injury the rats were injected with (3)H-thymidine and killed 4 hr later. Brain sections were processed for BSI-B4 isolectin histochemistry(More)
Traumatic brain injury (TBI) induces inflammatory reactions, and one of the essential mediators of this reaction is nitric oxide (NO). The action of this compound is still under study because no clear consensus has been reached about its exact action in the central nervous system. Further, it is unknown if, in the damaged brain, its neuroprotective activity(More)
Newborn, 2-, 4-, 8-, 16- and 30-day-old Wistar rats were injected with 3H-thymidine and sacrificed following 4 h. survival time. Brain sections containing the dorsal part of hippocampal formation were immunostained for S-100beta protein and subjected to autoradiography to visualize proliferating astrocytes. Microscopical observations revealed age-dependent(More)
Nitric oxide is an important mediator of inflammation in the brain, but it still remains unresolved whether its action is protective or not. In particular, it seems crucial to compare the effects observed in the mature brain with the developing brain of newborn animals. The influence of NO on tissue depends significantly on its concentration. In the present(More)