Marcus Hoffmann

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PURPOSE To investigate whether application of three all-in-one self-etching adhesives in a phase-separated stage has an adverse effect on bonding to enamel and dentin. MATERIALS AND METHODS The shear bond strengths (SBSs) on ground enamel and dentin of the adhesives G-Bond, Hybrid Bond, and iBond, combined with Venus composite, were determined. The(More)
BACKGROUND Methane yield and biogas productivity of biogas plants (BGPs) depend on microbial community structure and function, substrate supply, and general biogas process parameters. So far, however, relatively little is known about correlations between microbial community function and process parameters. To close this knowledge gap, microbial communities(More)
Age and sex dependence of subclass specific immunoglobulin G (IgG) Fc N-glycosylation was evaluated for 1709 individuals from two isolated human populations. IgGs were obtained from plasma by affinity purification using 96-well protein G monolithic plates and digested with trypsin. Fc N-glycopeptides were purified and analyzed by negative-mode MALDI-TOF-MS(More)
Site-specific glycosylation analysis is key to investigate structure-function relationships of glycoproteins, e.g. in the context of antigenicity and disease progression. The analysis, though, is quite challenging and time consuming, in particular for O-glycosylated proteins. In consequence, despite their clinical and biopharmaceutical importance, many(More)
Human fibrinogen is a 340 kDa, soluble plasma glycoprotein composed of paired sets of three subunits (α, β, γ). The protein plays a crucial role in protecting the vascular network against the loss of blood after tissue injury. The beta and gamma subunits each contain one N-glycosylation site, each of which is occupied by a biantennary N-glycan. So far(More)
The enormous challenges of mass spectrometry-based metaproteomics are primarily related to the analysis and interpretation of the acquired data. This includes reliable identification of mass spectra and the meaningful integration of taxonomic and functional meta-information from samples containing hundreds of unknown species. To ease these difficulties, we(More)
PURPOSE To evaluate the bond strengths on enamel and dentin with a self-etch adhesive (iBond), with or without oxygen-inhibited surface layer, or covered with intermediate self-curing resin, in combination with chemical-cured composite (Core Paste). METHODS Bond strengths on human enamel and dentin (n = 8) were determined according to the following(More)
For MALDI analysis of glycans and glycopeptides, the choice of matrix is crucial in minimizing desialylation by mass spectrometric in-source and metastable decay. Here, we evaluated the potential of 4-chloro-α-cyanocinnamic acid (Cl-CCA) for MALDI-TOF-MS analysis of labile sialylated tryptic N-glycopeptides and released N- and O-glycans. Similar to DHB, but(More)
PURPOSE To determine effects of light curing of self-etching adhesives under ambient air or nitrogen on inhibition depths, enamel and dentin bond strengths, and marginal adaptation in dentin cavities. MATERIALS AND METHODS The adhesives investigated were: AQ Bond (AQB, Sun Medical; Kyoto, Japan), iBond (IBO, Heraeus Kulzer; Hanau, Germany), One-Up Bond F(More)
OBJECTIVE Aim of this study was to design HEMA-free all-in-one self-etch model adhesives without phase separation, and to investigate their efficiency on extracted human teeth. MATERIALS AND METHODS Compositions of adhesives in mass% (1): UDMA (25), 4-META (20), H(2)O (0, 1, 2, 5, 10, 20, 35, and 45), balance of acetone or ethanol. (2): UDMA (35), 4-META(More)