Marco Severini

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By means of limited proteolysis of Bacillus stearothermophilus initiation factor IF2 and genetic manipulation of its structural gene, infB, we have been able to produce (or hyperproduce) and purify two polypeptide fragments corresponding to two structurally and functionally separate domains of the protein. The first is the G-domain (approximately 41 kDa),(More)
In the absence of ribosomes, Bacillus stearothermophilus translation initiation factor IF2 (Mr = 82 kDa) and its GTP-binding domain (i.e. the G-domain, Mr = 41 kDa) promote barely detectable hydrolysis of GTP. Upon addition of some aliphatic alcohols, however, the rate of nucleotide cleavage is substantially increased with both IF2 and G-domain, the highest(More)
The main problem in dealing with energy-harvesting (EH) sensor nodes is represented by the scarcity and non-stationarity of powering, due to the nature of the renewable energy sources. In this work, the authors address the problem of task scheduling in processors located in sensor nodes powered by EH sources. Some interesting solutions have appeared in the(More)
Translation initiation factor IF2 from Bacillus stearothermophilus (741 amino acids, Mr = 82,043) was subjected to trypsinolysis alone or in the presence of GTP. Following electroblotting and automated amino acid sequencing of the resulting peptides, the location and the sequential order of the main cleavage sites were identified. Trypsinolysis of IF2(More)
Overexpression of the porcine LH receptor (pLHR) ectodomain has been achieved using the baculovirus-insect cell system but mostly in an aggregated form with no secretion. In order to carry this out, new baculoviruses were selected to produce the pLHR ectodomain in insect Sf9 cells and caterpillars. In pLHR-P10-297 and pLHR-mel-319 baculoviruses, pLHR cDNA(More)
Porcine LH receptor ectodomain was overexpressed in insect cells and lepidopteran larvae using the recombinant baculovirus expression system. A low multiplicity of infection yielded the largest active production, of approximately 10(7) receptors/cell or 3 micrograms active receptor/mg total protein in infected cells. The truncated ectodomain solubilized(More)
In this paper, a novelty detection algorithm for the identification of leakages in smart water/gas grid contexts is proposed. It is based on two separate stages: the first deals with the creation of the statistical leakage-free model, whereas the second evaluates the eventual occurrence of leakage on the basis of the model likelihood. Up to the authors'(More)
Within micro grid scenarios, optimal energy management represents an important paradigm to improve the grid efficiency while lowering its burden. While usually real time energy management is considered, an offline approach can be also adopted to maximize the grid efficiency in certain contexts. Indeed, by evaluating the energy management performance(More)