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The ADH-induced water fluxes and the associated appearance of intramembranous particle aggregates in the luminal membrane of frog urinary bladders have been correlated in a time course study. Plots of the onset and reversal of the oxytocin-induced hydrosmotic response were sigmoidal in shape, symmetrical and slowed by low temperature to the same degree.(More)
The pumilio (pum) gene plays an essential role in embryonic patterning and germline stem cell (GSC) maintenance during oogenesis in Drosophila. Here we report on a phenotypic analysis using pum(ovarette) mutations, which reveals multiple functions of pum in primordial germ cell proliferation, larval ovary formation, GSC division, and subsequent oogenic(More)
Volume changes were studied in Beta vulgaris storage root vacuoles, using video microscopy, when exposed to hypotonic conditions. The osmotic gradient was either step-applied or progressively imposed in perfusion experiments. Preincubation at low pH (6.6) or with HgCl2 strongly reduced the vacuoles' water permeability, measured in step experiments.(More)
Mucosal acidification (from pH 8.1 to 6.0) reversibly inhibited the hydroosmotic responses to oxytocin, cyclic AMP and 8-bromo-cyclic AMP in frog urinary bladder. These inhibitory effects were only observed in the presence of a permeant buffer in the apical medium and could also be elicited by CO2 bubbling, even when the mucosal pH was clamped at 8.1. Acid(More)
To determine the accuracy and reliability of a labeled leukocyte imaging technique in the early diagnosis of pediatric appendicitis, we prospectively studied patients presenting to the pediatric emergency department with abdominal pain suggestive of appendicitis. Patients scheduled for urgent laparotomy were excluded, as were postmenarchal females. Blinded(More)
The existence of retinopetal neurons in the rat was verified using a morphological and neurochemical approach. Horseradish peroxidase injected into the posterior chamber of the eye labeled polygonal, ovoid, fusiform and small multipolar neurons in the lateral cell groups of the dorsal raphe nucleus. Very small electrolytic lesions of this region produced(More)
The present work aimed to determine whether GnRH potentiates the effect of growth hormone (GH) on insulin-like growth factors (IGF-I and IGF-II) hepatic gene expression in Sparus aurata liver. Since several hepatic genes were shown to underlie direct regulation via the hepatic estrogen receptor, another aim was to extend our understanding of direct estrogen(More)
The present work studied the effects of epidermal growth factor (EGF) on the release of thyrotropin (TSH) and prolactin (PRL) from perifused pituitary glands of 200-gram male Wistar rats. Each pituitary gland, cut into halves, was placed in a chamber of a perifusion system connected to a peristaltic pump which conveyed the perifusion medium (Medium 199, pH(More)
Human amnion was mounted, immediately after delivery, as a diaphragm between two lucite chambers and the net transepithelial water movement (Jw) was recorded minute by minute. When Jw was plotted against the applied transepithelial hydrostatic pressure (fetal side positive), in the absence of any other gradient, a linear relationship was observed (Phydr =(More)
The cloning and molecular characterization of water channels, generically called aquaporins, have marked a pivotal point in our understanding of water movements across epithelial barriers. Nevertheless, the mechanisms underlying water transfer across these barriers at the molecular and cellular level are not yet clarified. We analyze here the role of the(More)