Marcela Zamudio-Maya

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A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE(More)
AIMS To identify lactic acid bacteria (LAB) colonies isolated from sediments of a coastal marsh by the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) in MRS medium. METHODS AND RESULTS Single colonies isolated from sediments of a coastal marsh by enrichment in MRS broth were selected from MRS-TTC plates and classified according to colony phenotype(More)
The activities of primary ammonia assimilation enzymes were analyzed in isolated placentas of habanero peppers (Capsicum chinense Jacq.). The placentas were cultured in vitro and exposed to conditions promoting capsaicinoid accumulation, such as treatments with salicylic acid (SA) and methyl jasmonate (MeJa). Although exposure to both inducers resulted in(More)
AIM Development of a method for the isolation and purification of metagenomic RNA (mgRNA) from the ectopic bacterial flora of octopus. METHODS AND RESULTS Modifications were made to the methods of Valenzuela-Avendaño et al. (Plant Mol Biol Rep, 2005, 23, 199a) and Chomczynski and Sacchi (Anal Biochem, 1987, 162, 156) to develop a protocol based on(More)
Coastal bodies of water formed by the combination of seawater, underground rivers and rainwater comprise the systems with the greatest solar energy flow and biomass production on the planet. These characteristics make them reservoirs for a large number species, mainly microorganisms. Bacteria of the genus Vibrio are natural inhabitants of these environments(More)
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