Marcantonio Bragadin

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It is well established that cobalt mediates the occurrence of oxidative stress which contributes to cell toxicity and death. However, the mechanisms of these effects are not fully understood. This investigation aimed at establishing if cobalt acts as an inducer of mitochondrial-mediated apoptosis and at clarifying the mechanism of this process. Cobalt, in(More)
The rate of aerobic Ca2+ transport is limited by the rate of the H+ pump rather than by the Ca2+ carrier. The kinetics of the Ca2+ carrier has therefore been studied by using the K+ diffusion potential as the driving force. The apparent Vmax of the Ca2+ carrier is, at 20 degrees C, about 900 nmol (mg of protein)-1 min-1, more than twice the rate of the H+(More)
Thallium induces swelling in mitochondria and apoptosis in Jurkat cells. Both the swelling and the apoptosis are inhibited by means of cyclosporine A (CsA), thus suggesting that these phenomena result from the opening of the membrane transition pore (MTP) in mitochondria. Therefore, apoptosis can be explained as due to the opening of the MTP in mitochondria.
Tacrine (THA) and physostigmine (PHS) have been used in Alzheimer's disease therapy for their anticholinesterasic activity. Here we show that THA is taken up in rat lysosomes in an energy-dependent manner, and that it is also accumulated in acidic compartments of rat thymocytes and neuroblastoma cells. A concentration of THA less than 1 mM dissipated the pH(More)
The interactions of the tributyl, triethyl and trimethyllead compounds with energized mitochondria have been investigated in this paper. It has been shown that the (alkyl)(3)Pb-Cl compounds induce swelling in mitochondria suspended in a sucrose medium. The phenomenon is more marked the higher the lipophilicity and occurs in the following order:(More)
Melatonin, a metabolic product of the amino acid tryptophan, induces a dose-dependent energy drop correlated with a decrease in the oxidative phosphorylation process in isolated rat liver mitochondria. This effect involves a gradual decrease in the respiratory control index and significant alterations in the state 4/state 3 transition of membrane potential(More)
The interactions of tributyllead with lysosomes from rat liver have been studied. It results that the organometal compound induces a fast alkalinization in energized lysosomes. The interpretation is that the compound is a potent proton carrier. This function could explain the toxicity, in particular at neurological level of the compound.
1Dipartimento di Scienze Ambientali, Università di Venezia, DD 2137 30123 Venice, Italy 2Dipartimento di Chimica Inorganica, Metallorganica ed Analitica, Università di Padova, Via Marzolo 1, 35131 Padua, Italy 3Dipartimento di Biologia, Via U. Bassi 58/b, Università di Padova, 35121 Padua, Italy 4Dipartimento di Chimica Biologica, Università di Padova,(More)
OBJECTIVE Possibly due to a deficiency of insulin mediators, polycystic ovary syndrome (PCOS) is often associated with insulin resistance (IR) and hyperinsulinemia, likely responsible for an elevated production of reactive oxygen species. We investigated oxidative-related alterations in erythrocytes and anti-inflammatory effects of inositol in women with(More)
After the definitive ban on tin-based antifouling substances, new organic compounds have recently been introduced in antifouling paint formulations, as either principal or booster biocides. In most cases, previous risk assessment of these biocides has been inadequate so that their possible effects on aquatic ecosystems is a matter of great concern. We(More)