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Noncovalent, extrinsic fluorescent dyes are applied in various fields of protein analysis, e.g. to characterize folding intermediates, measure surface hydrophobicity, and detect aggregation or fibrillation. The main underlying mechanisms, which explain the fluorescence properties of many extrinsic dyes, are solvent relaxation processes and (twisted)(More)
The fluorescent dye Nile red was used as a probe for the sensitive detection of large, denatured aggregates of the model protein beta-galactosidase (E. coli) in solution. Aggregates were formed by irreversible heat denaturation of beta-galactosidase below and above the protein's unfolding temperature of 57.4 degrees C, and the presence of aggregates in(More)
The HERMES experiment is investigating the spin structure of the proton and neutron via deep{inelastic scattering of polarized positrons from polarized nuclear targets. The polarized positrons are provided by the HERA positron storage ring at DESY, Hamburg, Germany. The targets are pure internal gas targets. Data acquisition began in 1995, utilizing a(More)
We present the first precision measurement of the spin-dependent asymmetry in the threshold region of 3He(e,e') at Q2 values of 0.1 and 0.2 (GeV/c)2. The agreement between the data and nonrelativistic Faddeev calculations which include both final-state interactions and meson-exchange current effects is very good at Q2 = 0.1 (GeV/c)2, while a small(More)
Parameters presently used to analyse evoked potentials such as amplitude and latency are based on well defined single components, the recognition of which may become arbitrary in severely altered responses. Furthermore, they ignore changes also present in the remainder of an evoked potential wave form. Hence, they are ill suited for monitoring comatose(More)
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