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Extracellular Electron Transport-Mediated Fe(III) Reduction by a Community of Alkaliphilic Bacteria That Use Flavins as Electron Shuttles
TLDR
It is demonstrated that an alkaliphilic consortium dominated by Tissierella, Clostridium, and Alkaliphilus spp.
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Diffusion properties of single FoF1-ATP synthases in a living bacterium unraveled by localization microscopy
FoF1-ATP synthases in Escherichia coli (E. coli) bacteria are membrane-bound enzymes which use an internal protondriven rotary double motor to catalyze the synthesis of adenosine triphosphate (ATP).…
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3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria
TLDR
The one-dimensional diffusion coefficient of FoF1-ATP synthase diffusing on the long axis in living E. coli cells is obtained and the limited size of the observation area in the membrane with its significant membrane curvature has to be considered.
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Monitoring single membrane protein dynamics in a liposome manipulated in solution by the ABELtrap
TLDR
A combination of the ABELtrap with confocal FRET measurements to monitor single membrane enzyme dynamics by FRET for more than 10 seconds in solution and evaluates two different methods to trap the enzyme inside the confocal volume in order to extend the observation times.
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Monitoring subunit rotation in single FRET-labeled FoF1-ATP synthase in an anti-Brownian electrokinetic trap
TLDR
FoF1-ATP synthase is the membrane protein catalyzing the synthesis of the 'biological energy currency' adenosine triphosphate (ATP) and single-molecule Förster resonance energy transfer (FRET) is applied to monitor subunit rotation in the two coupled motors F1 and Fo.
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Device, system and method for illumination of a target area
TLDR
The illumination device (6) or the illumination system (2) for illuminating a surface of an interior of a vehicle as the target surface (4a, b) and the deflection unit (12) for changing the shape and intensity distribution in the second cone of light.
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