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NC-1 and HNK-1, two mouse monoclonal antibodies raised against quail ciliary ganglion and a human leukemic cell-line, respectively, were found to display the same pattern of reactivity. Species investigated included human, rodents, birds and amphibians. In 1- to 3-day-old avian embryos, migrating crest cells are stained, whereas in older animals(More)
Three transplantable nasopharyngeal carcinoma (NPC) tumors, designated C15, C17 and C18, have been obtained and characterized. C15, derived from a primary NPC tumor, has been propagated in nude mice for 30 passages. C17 and C18, derived from metastatic NPC tissue, have been passaged 10 times. Desmosomes, present in every case, provided confirmation of the(More)
The reactivity of the HNK-1 monoclonal antibody that, besides a subset of hematopoietic cells with natural killer activity, also detects neuroectodermal cells, was screened on 153 human tumors. Immunoperoxidase staining of paraffin sections revealed that HNK-1 detects neuroectoderm-derived cancers and tumors with endocrine activity. Because the antigen(More)
Cellular senescence is an irreversible proliferation arrest triggered by short chromosome telomeres, activated oncogenes, and cell stress and mediated by the pRB and p53 tumor suppressor pathways. One of the earliest steps in the senescence program is translocation of a histone chaperone, HIRA, into promyelocytic leukemia (PML) nuclear bodies. This(More)
Proteins with seven transmembrane segments (7TM) define a superfamily of receptors (7TM receptors) sharing the same topology: an extracellular N-terminus, three extramembranous loops on either side of the plasma membrane, and a cytoplasmic C-terminal tail. Upon ligand binding, cytoplasmic portions of the activated receptor interact with heterotrimeric(More)
Substrates of cyclin-cdk2 kinases contain two distinct primary sequence motifs: a cyclin-binding RXL motif and one or more phosphoacceptor sites (consensus S/TPXK/R or S/TP). To identify novel cyclin-cdk2 substrates, we searched the database for proteins containing both of these motifs. One such protein is human HIRA, the homologue of two cell(More)
The number of D4Z4 repeats in the subtelomeric region of chromosome 4q is strongly reduced in patients with Facio-Scapulo-Humeral Dystrophy (FSHD). We performed chromosome conformation capture (3C) analysis to document the interactions taking place among different 4q35 markers. We found that the reduced number of D4Z4 repeats in FSHD myoblasts was(More)
The HNK-1 antibody known to define a subpopulation of human lymphocytes with natural killer and killer cell activities was shown to detect a common neuroectodermal antigen. Most tumor lines and paraffin-embedded tumors and normal tissues of neuroectodermal origin were specifically stained by HNK-1. Lines and tissues of other derivations were negative except(More)
Natural cell-mediated cytotoxicity (NCMC) against K-562 target cells was explored in patients with various primary immunodeficiencies. (a) NCMC was present in 2 patients lacking detectable B cells. Conversely, NCMC was depressed in 2 patients with severe combined immunodeficiency presenting a normal number of circulating B cells. These data exclude a major(More)
Natural killer (NK) cell activity was found decreased (mean value: 27.5%) in peripheral blood lymphocytes from 59 untreated patients with malignant lymphoma, when compared to 112 healthy subjects (46.3%) and to 75 cancer patients with nonlymphoid tumors (43.7%) (P less than 0.001). NK cell values were similar in patients with Hodgkin's disease (25.5%) and(More)