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INTRODUCTION Cytokines are humoral regulatory molecules that act together in immunologic pathways. Monitoring cytokines and their variations within physiologic ranges is critical for biomarker discovery. Therefore, we evaluated the performance characteristics of 72 analytes measured by multiplex cytokine immunoassay, with an emphasis on the differences of(More)
Standardization of immunophenotyping requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center comparison of data. Experts developed five standardized, eight-color panels for identification of major immune cell subsets in peripheral blood. These were produced as(More)
BACKGROUND AND OBJECTIVES Platelet additive solutions (PAS) have been shown to be suitable for extended platelet storage but have required the carryover of substantial (30%) amounts of plasma for success. Improving platelet quality by optimizing the composition of PAS may allow a reduction to be made in the amount of plasma carried over. Reducing the(More)
BACKGROUND There is often a need in flow cytometry to display and analyze histograms at resolutions lower than those native to the data. It is common, for example, to analyze DNA histograms at 256-channel resolution, even though the data were acquired at 1,024 channels or more. The most common method for reducing resolution, referred to as the consecutive(More)
BACKGROUND Cytokines are humoral molecules that elicit regulatory function in immunologic pathways. The level and type of cytokine production has become critical in distinguishing physiologic from pathologic immune conditions. Cytokine profiling has become an important biomarker discovery tool in monitoring of the immune system. However, the variations in(More)
Protein from detergent-disrupted feline leukemia virus was found to suppress lymphocyte blastogenic responsiveness of peripheral blood mononuclear cells from normal cats. When suppressive doses of this protein were substituted for mitogen in the preactivation step of the concanavalin A-induced suppressor cell assay, significant suppression was observed in(More)
Preactivation of feline peripheral blood mononuclear cells with mitogenic doses of concanavalin A resulted in suppression of proliferation when these cells were cocultured with untreated autologous cells. The degree of suppression was directly proportional to the preactivation time and ratio of preactivated to untreated cells. The generation, but not the(More)
The in vitro effect of vitamin E and 3 other antioxidants--ethoxyquin, 2-mercaptoethanol, and ascorbic acid--on proliferation of canine lymphocytes was examined. Lymphocytes from 2 groups of dogs given a vitamin E-deficient diet or whelped from a bitch fed such a diet were cultured with pooled samples of serum from dogs fed a vitamin E-deficient diet or(More)
A 3-methylcholanthrene [(MCA) CAS: 56-49-5]-induced fibrosarcoma cell line and its Friend murine leukemia virus-infected counterpart were assessed for their susceptibility to lysis by so-called "natural" effector cells in a series of 51Cr release assays. Detailed functional and phenotypic analysis of lytic effector cell populations from normal C57BL/6 mouse(More)