María Julia Arcos-Martínez

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This work reports monoamine oxidase (MAO)/horseradish peroxidase (HRP) and diamine oxidase (DAO)/horseradish peroxidase (HRP) based biosensors using screen-printed carbon electrodes for the determination of biogenic amines (BA). The enzymes have been covalently immobilized onto the carbon working electrode, previously modified by an aryl diazonium salt,(More)
Disposable screen-printed biosensors have been successfully employed in the development of analytical methods that respond to the growing need to perform rapid "in situ" analyses. Thus, the early detection of microorganisms, which plays an important role in the prevention of human health problems, animals and plants epidemics, has been carried out using(More)
Carbon screen-printed electrodes (CSPEs) modified with metal nanoparticles present an interesting alternative in the determination of chromium(VI) by differential pulse voltammetry (DPV). Metallic silver and gold nanoparticle deposits have been obtained by electrochemical deposition. Scanning electron microscopy measurements show that the electrochemically(More)
This work summarizes the manufacturing procedure of Horseradish peroxidase (HRP) based biosensors for the determination of the mycotoxin Ochratoxin A (OTA). The biosensors have been fabricated using the single technology of screen-printing. That is to say, an HRP containing ink has been directly screen-printed onto carbon electrodes, which offers a higher(More)
This work presents the simultaneous determination of cadaverine, histamine, putrescine and tyramine by square wave voltammetry using a boron-doped diamond electrode. A multivariate calibration method based on partial least square regressions has allowed the resolution of the very high overlapped voltammetric signals obtained for the analyzed biogenic(More)
An easy covalent immobilization method used to develop enzyme biosensors based on carbon and gold screen printed electrodes (SPCEs and gold SPEs) is described. The linkage of biomolecules through 4-nitrobenzenediazonium tetrafluoroborate, mercaptopropionic acid and thioctic acid monolayers has been attempted using bare SPCEs and gold SPEs, as well as gold(More)
Enzymatic amperometric procedures for measurement of Hg (II), based on the inhibitive action of this metal on urease enzyme activity, were developed. Screen-printed carbon electrodes (SPCEs) and gold nanoparticles modified screen-printed carbon electrodes (AuNPs/SPCEs) were used as supports for the cross-linking inmobilization of the enzyme urease. The(More)
Horseradish peroxidase has been successfully immobilized in a polypyrrole matrix onto disposable screen-printed carbon electrodes for the selective detection of Ochratoxin A. The chronoamperometric determination of this mycotoxin has been optimized by experimental design methodology, which implied the join evaluation of pH of the buffer solution, applied(More)
Enzymatic amperometric procedures for measuring arsenic, based on the inhibitive action of this metal on acetylcholinesterase enzyme activity, have been developed. Screen-printed carbon electrodes (SPCEs) were used with acetylcholinesterase covalently bonded directly to its surface. The amperometric response of acetylcholinesterase was affected by the(More)
This work shows an easy and fast electrochemical method for Levetiracetam (LEV) determination, which is a novel antiepileptic. Most of the methods used up to now for its determination required a pre-treatment of the sample. It is shown here that the developed Peroxidase based biosensors avoid this kind of drawbacks. Screen-printed carbon electrodes have(More)