María José Alejandre

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Chick liver and intestine 3-hydroxy-3-methylglutaryl-CoA reductase did not show diurnal rhythm at hatching. Differences in activity between light and dark periods appeared during the first week and remained more or less constant between 10-14 days after hatching. Hepatic and intestinal reductase activities were maximal during the light period and minimal(More)
The developmental pattern of microsomal 3-hydroxy-3-methylglutaryl-CoA reductase activity was different in liver, intestine and brain of neonatal chicks. Hepatic reductase activity sharply increased between 5 and 9 days after hatching. This pattern agrees with changes in acetate incorporation into non-saponifiable lipids by liver slices. Both enzyme(More)
Both 5% cholesterol feeding and fasting produced a decrease in the hepatic 3-hydroxy-3-methylglutaryl-CoA reductase activity, although certain diurnal variations remained during the second day of treatment. Supplementation of 5% cholesterol to the diet produced a significant increase in cholesterol content of hepatic microsomes, whereas no significant(More)
The Arrhenius plots of hepatic and cerebral 3-hydroxy-3-methylglutaryl-CoA reductase activity were studied in neonatal chicks fed with a standard diet. Supplementation of the diet with 2% cholesterol from hatching has no effect on the thermal characteristics of the brain enzyme. The Arrhenius plot of brain reductase was practically similar to that found in(More)
OBJECTIVES Pancreatic ductal adenocarcinoma remains as a chemoresistant disease with the poorest prognosis. Gemcitabine has been the standard treatment during the last decade. Erlotinib, a tyrosine kinase inhibitor, in combination with gemcitabine produces a small increase in survival. However, these results remain insufficient. The aim of this study was to(More)
The overall survival of patients with pancreatic ductal adenocarcinoma is extremely low. Although gemcitabine is the standard used chemotherapy for this disease, clinical outcomes do not reflect significant improvements, not even when combined with adjuvant treatments. There is an urgent need for prognosis markers to be found. The aim of this study was to(More)
Smooth muscle cells (SMCs) undergo changes related to proliferation and apoptosis in the physiological remodeling of vessels and in diseases such as atherosclerosis and restenosis. Recent studies also have demonstrated the vascular cell proliferation and programmed cell death contribute to changes in vascular architecture in normal development and in(More)
We have studied the correlation between changes in the lipid composition in chick liver microsomes and the activities of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) and acyl-CoA: cholesterol acyltransferase (ACAT) by in vivo and in vitro experiments with 21-day-old chicks. A 5% cholesterol diet for 3 hr produced an increase in the(More)
OBJECTIVE Pancreatic ductal adenocarcinoma is a deadly disease because of late diagnosis and chemoresistance. We aimed to find a panel of serum cytokines representing diagnostic and predictive biomarkers for pancreatic cancer. METHODS A cytokine antibody array was performed to simultaneously identify 507 cytokines in sera of patients with pancreatic(More)
The beta-lactone isolated from Fusarium sp. termed L-659,699 is a potent specific inhibitor of the enzyme 3-hydroxi-3-methylglutaril coenzyme A (HMG-CoA) synthase. In cultures of smooth muscle cells (SMC) isolated from aortic-arch of control (C-SMC) and 5% of cholesterol diet (Ch-SMC) treated chicks, the incorporation of (14C)-acetate to lipids(More)