María Elena De Tomás

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The incorporation of 1-14C-linoleic acid, 1-14C-α-linolenic acid and 1-14C-arachidonic acid into rat liver lipids was measured and the per cent distribution of radioactivity into the different lipid fractions determined. Normal rats were injected into the portal vein with the labeled solutions during a one minute period. Livers were quickly frozen,(More)
The effects of the n−3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the growth of a human lung mucoepidermoid carcinoma (HLMC) in athymic mice were studied. The mice were divided into three groups which were given either a control chow diet (C), a chow diet supplemented with EPA/DHA (P) (25 or 50 mg of free n−3 fatty acids/g(More)
Pregnant rats were kept throughout gestation on a control diet (i.e., 25% protein), on a low protein diet (i.e., 5% protein) or on a fat-free diet. At 20–21 days of gestation, the rate of 9-, 6-, and 5-desaturation was measured, using microsomes from maternal and fetal livers and placenta microsomes. The effect of protein malnutrition was more evident upon(More)
The Δ9 desaturase activity and the14C radioactivity of the de novo synthesized fatty acids incorporated into microsomal lipids and serum triglycerides were measured under different nutritional conditions. The results obtained indicate a correlation between the values of the three parameters studied after starvation or after refeeding Purina chow or either a(More)
The effect of protein depletion in the pregnant rat on the polyunsaturated fatty acid incorporation into very low density lipoproteins (VLDL) has been investigated. The apoprotein pattern of these particles was determined. In in vivo experiments the amounts of serum and liver triacylglycerol were determined. VLDL were isolated and their apo C concentration(More)
Essential fatty acid (EFA) deficient rats were injected intraportally with a solution of 1-14C-linoleic acid during a 1 min period. Livers were quickly frozen, pulverized, and the lipids extracted and fractioned by thin layer chromatography. The incorporation of 1-14C-linoleic acid into liver lipids was measured. The results were compared with those(More)
The activity of Δ9 desaturase was determined in the microsomal fraction of normal mouse liver and hepatoma SS1K in the presence of the 105,000 × g supernatant. Neither hepatic nor hepatoma soluble fractions were able to modify the low desaturating capacity. Two enzymes from the microsomal electron transport chain associated with Δ9 desaturase, namely(More)
Essential fatty acid (EFA)-deficient rats were injected intraportally with a labeled solution containing3H arachidonic acid and14C-linoleic acid during a 1 min period. Livers were quickly frozen, pulverized, and the lipids extracted and fractioned by thin layer chromatography. The incorporation of3H and14C into liver lipids was measured, and the per cent(More)
The effect of a low protein diet during pregnancy and lactation on the fatty acid composition of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) from brains of ten-day-old rats was studied. The results indicated that partial deprivation of protein during early development was associated with an increase in the fatty acids of the n−9 family in PC.(More)
In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chromatography. These results proved that Ehrlich ascites cells(More)