Manuel A F V Gonçalves

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The initial discovery of adeno-associated virus (AAV) mixed with adenovirus particles was not a fortuitous one but rather an expression of AAV biology. Indeed, as it came to be known, in addition to the unavoidable host cell, AAV typically needs a so-called helper virus such as adenovirus to replicate. Since the AAV life cycle revolves around another(More)
Effective gene therapy is dependent on safe gene delivery vehicles that can achieve efficient transduction and sustained transgene expression. We are developing a hybrid viral vector system that combines in a single particle the large cloning capacity and efficient cell cycle-independent nuclear gene delivery of adenovirus (Ad) vectors with the long-term(More)
Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene, making it a potential target for gene therapy. There is, however, a scarcity of vectors that can accommodate the 14-kb DMD cDNA and permanently genetically correct muscle tissue in vivo or proliferating myogenic progenitors in vitro for use in autologous transplantation. Here, a dual(More)
Viral gene carriers are being widely used as gene transfer systems in (trans)differentiation and reprogramming strategies. Forced expression of key regulators of pancreatic differentiation in stem cells, liver cells, pancreatic duct cells, or cells from the exocrine pancreas, can lead to the initiation of endocrine pancreatic differentiation. While several(More)
DESCRIPTION An 83-year-old woman was admitted to the emergency department reporting a 3-day progressively worsening lumbar pain and fever. Her past medical history was remarkable for type 2 diabetes mellitus, class 3 chronic renal failure, repetitive urinary tract infections and hypothyroidism. On admission, the patient was pale, febrile with arterial(More)
SUMMARY Neurochemical Aspects of Alzheimer's Disease Alzheimer's Disease is one of the most severe and most common progressive neurodegenera tive diseases of ageing. It accounts for a signiflcant number of cases of senile dementia. It is a major health problem, considering the current increase in the geriatric population all over the world. The purpose of(More)
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