Manqing Huang

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The gene yesN encodes a putative carbohydrate utilization response regulator in Lactobacillus plantarum. In order to determine the regulatory function of the yesN in L. plantarum, a yesN null mutant strain, L. plantarum ΔyesN, was constructed and confirmed by PCR and sequencing analyses. Using glucose, sucrose, and inulin as the sole carbon source, the(More)
The C-terminus of the putative cell surface protein CspI which contains one putative LPxTG motif region and a signal peptides fragment were amplified from L. plantarum CICC6024, and the green fluorescent protein gene gfp was amplified from the plasmid pACGFP. The three genes were ligated and the fusion gene was named SgfpL. The fusion gene SgfpL was then(More)
A method of high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the determination of thyroid hormones in milk including 3,3',5,5 '-tetraiodothyronine (T4), 3,3', 5-triiodothyronine (T3) and 3,3',5 '-triiodothyronine (rT3). The sample was extracted with acetonitrile and centrifuged, then the up layer was alkalized(More)
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