Manoj Kumar Mishra

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In the present study, Agrobacterium tumefaciens-mediated transformation protocol has been optimized for Jatropha curcas—an important biofuel plant using leaf segments (LS) and hypocotyl segments (HS) from in vitro-seedlings. Among different accessions used, ‘GJ-Ran-C2’ was selected as the best for transformation. A. tumefaciens strain EHA101, containing the(More)
BACKGROUND Sterol glycosyltrnasferases (SGT) are enzymes that glycosylate sterols which play important role in plant adaptation to stress and are medicinally important in plants like Withania somnifera. The present study aims to find the role of WsSGTL1 which is a sterol glycosyltransferase from W. somnifera, in plant's adaptation to abiotic stress. (More)
Sterol glycosyltransferases (SGTs) catalyze the transfer of sugar molecules to diverse sterol molecules, leading to a change in their participation in cellular metabolism. Withania somnifera is a medicinal plant rich in sterols, sterol glycosides and steroidal lactones. Sterols and their modified counterparts are medicinally important and play a role in(More)
Wireless mesh networks (WMNs) have attracted increasing attention and deployment as a high-performance and low cost solution to last-mile broadband Internet access. Wireless mesh networks have emerged as a potential technology for next-generation wireless networking. WMNs have been widely accepted in the traditional application sectors of ad hoc networks(More)
Wide implementation of IEEE 802.11 based networks could lead to deployment of localized wireless data communication environments with a limited number of mobile hosts, called ad hoc networks. Implementation of a proper routing methodology in ad hoc networks makes it efficient in terms of performance. A wide spectrum of routing protocols has been contributed(More)
Glycosylation of sterols, catalysed by sterol glycosyltransferases (SGTs), improves the sterol solubility, chemical stability and compartmentalization, and helps plants to adapt to environmental changes. The SGTs in medicinal plants are of particular interest for their role in the biosynthesis of pharmacologically active substances. WsSGTL1, a SGT isolated(More)
Electronic collaboration among devices in a geographically localized environment is made possible with the implementation of IEEE 802.11 based wireless ad hoc networks. Dynamic nature of mobile ad hoc networks(MANETs) may lead to unpredictable intervention of attacks or fault occurrence, which consequently may partition the network, degrade its performance,(More)
BACKGROUND Interstitial cystitis (IC), more recently called painful bladder syndrome (PBS) is a complex disease associated with chronic bladder inflammation that primarily affects women. Its symptoms include frequent urinary urgency accompanied by discomfort or pain in the bladder and lower abdomen. In the United States, eight million people, mostly women,(More)
Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), which is thought to result from immune-mediated inflammatory disorders, leads to high morbidity and health care cost. Fatty acid amide hydrolase (FAAH) is an enzyme crucially involved in the modulation of intestinal physiology through anandamide (AEA) and other(More)
The vertebrate immunological defense system relies upon interdependent regulatory interactions between the innate and adaptive immune compartments playing a pivotal role in various physiological and immunopathological conditions. Recent studies have demonstrated that interactions between dendritic cells and NK cells or T cells and NK cells are important for(More)