Manisha Parulekar

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BACKGROUND Mutation of the RB1 gene is necessary but not sufficient for the development of retinoblastoma. The nature of events occurring subsequent to RB1 mutation is unclear, as is the retinal cell-of-origin of this tumour. METHODS Gene expression profiling of 21 retinoblastomas was carried out to identify genetic events that contribute to tumorigenesis(More)
Treatment of newborn rat pups with 60 mg/kg.d chlorphentermine for 7 d produced on accumulation of alveolar foam cells accompanied by an increase in relative pulmonary tissue weight. In contrast, administration of 20 mg/kg.d for 1 wk did not markedly alter lung ultrastructure or weight in newborns. Both doses of chlorphentermine elevated the activity of(More)
Retinoblastoma is the most common malignant tumour of the eye in childhood, with nearly all bilateral tumours and around 17-18% of unilateral tumours due to an oncogenic mutation in the RB1 gene in the germline. Genetic testing in all cases enables accurate risk assessment and optimal clinical management for the affected individual, siblings, and future(More)
A homogenate of purified chromaffin cells was fractionated, after removal of the nuclear fraction, by sucrose density gradient ultracentrifugation. The presence and subcellular localization of low molecular mass GTP-binding proteins was explored by incubation of blots of proteins from different subcellular fractions with [alpha-32P]GTP in the presence of(More)
Daily, intraperitoneal administration of either vanadium pentoxide (VP) or sodium vanadate (NaV) at a dose of 1 mg/kg for 14 days produced no marked change in pulmonary protein, glycogen and phospholipid (PL) levels of adult male rats. Increasing the VP dose to 4 mg/kg decreased lung PL content, while an equivalent NaV dose elevated tissue glycogen.(More)
Three tetrachlorobenzene (TCB) congeners (1,2,3,4- 1,2,3,5-, and 1,2,4,5-) were administered daily by gavage to pregnant Sprague-Dawley rats at levels of 50, 100, or 200 mg/kg from day 6 through day 15 of gestation. Mothers were sacrificed on day 21 of gestation and the pups removed by cesarian section for teratological evaluation. Administration of(More)