Manish Kant Dubey

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Phosphonate-steel interactions have been industrially significant for decades, but details of the phosphonate-steel interface have not yet been characterized. Self-assembled monolayers of phosphonic acids were formed on stainless steel 316L by room-temperature solution deposition. The acids are covalently bound to the surface as phosphonates in a bidentate(More)
Improving the efficiency of enzymatic hydrolysis of cellulose is one of the key technological hurdles to reduce the cost of producing ethanol and other transportation fuels from lignocellulosic material. A better understanding of how soluble enzymes interact with insoluble cellulose will aid in the design of more efficient enzyme systems. We report a study(More)
The interaction of beta-cyclodextrin (beta-CD) with mixed bilayers composed of sphingomylein and cholesterol (Chol) above and below the accepted stable complexation ratio (67:33) was investigated. Membranes with the same (symmetric) and different (asymmetric) compositions in their inner and outer leaflets were deposited at surface pressures of 20, 30, and(More)
A study of the interaction of four endoglucanases with amorphous cellulose films by neutron reflectometry (NR) and quartz crystal microbalance with dissipation monitoring (QCM-D) is reported. The endoglucanases include a mesophilic fungal endoglucanase (Cel45A from H. insolens), a processive endoglucanase from a marine bacterium (Cel5H from S. degradans ),(More)
The misfolding and aggregation of the intrinsically disordered, microtubule-associated tau protein into neurofibrillary tangles is implicated in the pathogenesis of Alzheimer's disease. However, the mechanisms of tau aggregation and toxicity remain unknown. Recent work has shown that anionic lipid membranes can induce tau aggregation and that membrane(More)
We investigated hydroxyalkylphosphonate monolayers as a novel platform for the biofunctionalization of silicon-based field effect sensor devices. This included a detailed study of the thin film properties of organophosphonate films on Si substrates using several surface analysis techniques, including AFM, ellipsometry, contact angle, X-ray photoelectron(More)
Temperature-sensitive hydrogel polymers are utilized as responsive layers in various applications. Although the polymer's native characteristics have been studied extensively, details concerning its properties during interaction with biorelated structures are lacking. This work investigates the interaction between a thermoresponsive polymer cushion and(More)
by S. I. bohnenStengel, S. e. belcher, A. AIken, J. D. AllAn, g. Allen, A. bAcAk, t. J. bAnnAn, J. F. bArlow, D. c. S. beDDowS, w. J. bloSS, A. M. booth, c. cheMel, o. coceAl, c. F. DI MArco, M. k. Dubey, k. h. FAloon, Z. l. FleMIng, M. Furger, J. k. gIetl, r. r. grAveS, D. c. green, c. S. b. grIMMonD, c. h. hAlIoS, J. F. hAMIlton, r. M. hArrISon, M. r.(More)
Polymer-supported single lipid bilayers are models to study configurations of cell membranes. We used off-specular neutron scattering to quantify in-plane height-height correlations of interfacial fluctuations of such a lipid bilayer. As temperature decreased from 37 °C to 25 °C, the polymer swells and the polymer-supported lipid membrane deviates from its(More)
Nitric oxide (NO) is important physiologically as a gaseous messenger in the central nervous system;1 its level is also associated with several disease states.2 The concentration of NO in these cases is in the nanomolar range; therefore accurate and direct detection of NO at these levels is important. NO is also a decomposition product of several(More)