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Oxygen uptake and the mode of energy production in Sipunculus nudus L. were determined at different oxygen levels by means of direct and indirect calorimetry. Oxygen consumption declined linearly with decreasing ambient P Or A similar decrease in heat production was observed down to a Po 2 of 8.66 kPa. At lower oxygen tensions, a discrepancy between aerobic(More)
Octopine dehydrogenase (OcDH) from the adductor muscle of the great scallop, Pecten maximus, catalyzes the NADH dependent, reductive condensation of L-arginine and pyruvate to octopine, NAD(+), and water during escape swimming and/or subsequent recovery. The structure of OcDH was recently solved and a reaction mechanism was proposed which implied an ordered(More)
Opine dehydrogenases catalyze the reductive condensation of pyruvate with L-amino acids. Biochemical characterization of alanopine dehydrogenase from Arenicola marina revealed that this enzyme is highly specific for L-alanine. Unbiased molecular dynamics simulations with a homology model of alanopine dehydrogenase captured the binding of L-alanine diffusing(More)
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