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Lysogeny (bacteria containing inducible prophages) and lytic viral infection (bacteria in a lytic stage of infection) were investigated at the community level in contrasting marine environments such as estuarine versus offshore waters, surface versus deep waters, and oxic versus anoxic waters in the Mediterranean and Baltic Seas. The frequency of lysogenic(More)
The aim of this study was to develop a protocol for the simultaneous extraction from bacterioplankton of RNA and DNA suitable for quantitative molecular analysis. By using a combined mechanical and chemical extraction method, the highest RNA and DNA yield was obtained with sodium lauryl sarcosinate-phenol or DivoLab-phenol as the extraction mix. The(More)
High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4',6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic(More)
Identification and functional analysis of key members of bacterial communities in marine and estuarine environments are major challenges for obtaining a mechanistic understanding of biogeochemical processes. In the Baltic Sea basins, as in many other marine environments with anoxic bodies of water, the oxic-anoxic interface is considered a layer of high(More)
The bacterial core communities of bulk water and corresponding biofilms of a more than 20-year-old drinking water network were compared using 16S rRNA single-strand confirmation polymorphism (SSCP) fingerprints based on extracted DNA and RNA. The structure and composition of the bacterial core community in the bulk water was highly similar (>70%) across the(More)
27 Based on lipid analyses, 16S rRNA/rRNA gene single strand conformation polymorphism 28 fingerprints and methane flux measurements, influences of the fertilisation regime on 29 abundance and diversity of archaeal communities were investigated in soil samples from the 30 long-term (103 years) field trial in Bad Lauchstädt, Germany. The investigated plots(More)
BACKGROUND Routine clinical diagnostics of CF patients focus only on a restricted set of well-known pathogenic species. Recent molecular studies suggest that infections could be polymicrobial with many bacteria not detected by culture-based diagnostics. METHODOLOGY AND PRINCIPAL FINDINGS A large cohort of 56 adults with continuous antibiotic treatment was(More)
The taxonomic relationship between several Shewanella putrefaciens isolates from the Baltic Sea and reference strains of this species is presented in this study. Results from DNA-DNA hybridization using a newly developed non-radioactive detection system and from 16S rRNA gene sequencing demonstrated that S. putrefaciens is a heterogeneous species containing(More)
Community structure of bacterioplankton was studied during the major growth season for phytoplankton (April to October) in the epilimnion of a temperate eutrophic lake (Lake Plusssee, northern Germany) by using comparative 5S rRNA analysis. Estimates of the relative abundances of single taxonomic groups were made on the basis of the amounts of single 5S(More)
Two bacterial strains isolated from the Baltic Sea, OS145T and OS146, were characterized on the basis of their physiological and biochemical features, their fatty acid profiles and their phylogenetic position based on 16S rDNA sequence analyses. The strains were isolated from the upperoxic water column of the central Baltic Sea. Phylogenetic analyses of the(More)