Mandy G. Katz-Jaffe

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OBJECTIVE To evaluate a new strategy for comprehensive chromosome screening at the blastocyst stage. DESIGN Clinical research study. SETTING An IVF clinic and a specialist preimplantation genetic diagnosis laboratory. PATIENT(S) Forty-five infertile couples participated in the study. The mean maternal age was 37.7 years, and most couples had at least(More)
OBJECTIVE To identify and transfer cytogenetically normal embryos after screening all chromosomes of first and second polar bodies (PBs) or trophectoderm samples with the use of comparative genomic hybridization. DESIGN Clinical research study. SETTING In vitro fertilization clinic referring samples to a specialist preimplantation genetic diagnosis(More)
In contrast to the preimplantation mammalian embryo, it has been notoriously difficult to cryopreserve the metaphase II oocyte. The ability to store oocytes successfully at -196 degrees C has numerous practical and financial advantages, together with ethical considerations, and will positively impact animal breeding programs and assisted conception in the(More)
OBJECTIVE To assess correlation between blastocyst morphology and chromosomal status. DESIGN Observational research study. SETTING An IVF clinic and a specialist preimplanation genetic diagnosis (PGD) laboratory. PATIENT(S) Ninety-three couples undergoing IVF treatment in combination with chromosome screening of embryos. INTERVENTION(S) Five hundred(More)
OBJECTIVE To assess the potential benefit of preimplantation aneuploidy testing on the outcome of in vitro fertilization (IVF) for women of advanced maternal age (AMA). DESIGN Prospective randomized clinical trial. SETTING Private IVF clinic. PATIENT(S) Sixty-two infertile AMA couples undergoing fertility treatment. INTERVENTION(S) Fluorescent in(More)
OBJECTIVE To examine human blastocyst microRNA (miRNA) expression in correlation with human infertility. MicroRNAs are small, noncoding RNA molecules that regulate gene expression via mechanisms such as degradation and translational suppression of targeted messenger RNAs. Recent data has pointed to the importance of miRNAs in disease states and during mouse(More)
OBJECTIVE To analyze the protein production into the surrounding medium (secretome) of both human and mouse embryos and correlate these findings with ongoing blastocyst development. Along with improvements in culture systems, there is renewed focus on the development of noninvasive viability assays in human IVF. Because the majority of biologic functions(More)
The combination of trophectoderm biopsy, blastocyst vitrification, and single-nucleotide polymorphism microarray-based technology for comprehensive chromosome screening results in high implantation and live birth rates that could contribute to the practical application of single embryo transfer for infertility patients.
OBJECTIVE To investigate the impact of maternal aging on the molecular signature of cumulus cells. DESIGN Experimental study. SETTING Research laboratory. PATIENT(S) Patients, young fertile oocyte donors (n = 40) and infertile women of advanced maternal age (40-45 years; n = 48), donated, with Institutional Review Board consent, cumulus cells during(More)
OBJECTIVES To develop a reliable method to isolate fetal cells for genetic diagnosis. DESIGN Aspiration of cervical mucus from pregnant women in the first trimester. SETTING Pregnant women were recruited before an elective termination of pregnancy. POPULATION Sixty pregnant women (7-10 weeks of gestation). METHODS Fetal cells were isolated from(More)