Man Mohan Misro

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H(2)O(2) induces apoptosis in variety of cells; however, the sensitivities of testicular germ cells to H(2)O(2) are not known. In the present study, H(2)O(2), at concentrations in the range 1-10 microM, was found to induce apoptosis in testicular germ cells in vitro. Following 1 h of treatment with 10 microM H(2)O(2), a 10-fold rise in the percentage of(More)
In order to understand the pathogenesis of estradiol induced effects in the seminiferous epithelium, studies were undertaken in adult rats with estradiol-3-benzoate administered for different durations. After 30 d of treatment, a significant rise in lipid peroxidation with concomitant fall in the activities of superoxide dismutase and catalase was observed.(More)
The detrimental effects of persistent stimulation with hCG were investigated in rat Leydig cells in vitro. Significant rise in lipid peroxidation and reactive oxygen species (ROS) with concomitant attenuation in the activities of antioxidant enzymes: superoxide dismutase, catalase, and glutathione-S-transferase was observed. Transcripts for catalase and(More)
OBJECTIVE To determine whether or not sperm function parameters are altered in male partners of couples with a history of idiopathic recurrent pregnancy loss (RPL). DESIGN In comparison with proven fertile volunteers, sperm function parameters like hypo-osmotic swelling (HOS), acrosomal status (AS), and nuclear chromatin decondensation (NCD) were assessed(More)
AIM The data on semen analysis of subjects attending the Fertility Clinic at NIHFW (National Institute of Health and Family Welfare) Munirka, New Delhi for the last 11 years were analyzed to verify the claims and speculations on declining sperm counts in men. METHODS Approximately 10 % of the records every year starting from 1990 to 2000 (numbering 1176(More)
OBJECTIVE To evaluate various causes possibly contributing towards recurrent pregnancy loss (RPL), particularly male factors. Prospective study of 75 couples with history of RPL who were investigated for genetic, anatomic, immunological, infective and systemic causes in both partners. Functional sperm capacity was assessed by the Hypo-osmotic swelling test(More)
A new contraceptive technique has been proposed and tested. The method uses a novel polymer which when injected into the vas deferens, lowers the pH sufficiently kill the spermatozoa passing through. The polymer itself does not degrade in the process but can be flushed out to regain fertility. Experiments carried out on rats demonstrate the efficacy of the(More)
OBJECTIVE To investigate the association between specific reactive nitrogen species and sperm function among infertile subjects. DESIGN Controlled trial. SETTING Fertility clinic at the National Institute of Health and Family Welfare, New Delhi, India. PATIENT(S) Proven fertile volunteers and subjects attending the above clinic. INTERVENTION(S)(More)
Selective deprivation of gonadotrophins in prepubertal rats by administration of a GnRH antagonist (Ac-D2Nal1, D4ClPhe2, DTrp3, DArg6, DA1a10-GnRH; GnRH code: 103-289-10, National Institutes of Health, USA) for 3 weeks, initiated at 20-22 days of age, induced morphological changes in the Leydig cells, including thickening and indentation of the nuclear(More)
The present study was aimed to investigate the beneficial effects of N-acetyl-l-cysteine (NAC, 150 mg/kg bw twice/week) against testicular germ cell apoptosis in rats induced by chronic hCG administration (100 IU/rat/day for 30 days). NAC co-treatment improved serum testosterone, prevented rise in lipid peroxidation, intracellular H2O2 and the activities of(More)