Mamed Mustafaev

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Covalent bioconjugation between anionic polyelecrolytes and polypeptide antigens chemically synthesized by solid-phase chemistry, were studied in hydrated reversed micelle systems. The epitops of Foot-and-Mouse disease virus VP1 protein (40--60 and 135--160 residues) were used as polypeptide antigens. The polypeptide-comprising Biopolymer Systems were(More)
We have synthesized the 17beta-estradiol comprising polycomplexes by the Cu(2+)-mediated complex formation of polyanionic polyelectrolyte (PE), [polyacrylic acid (PAA), nontoxic copolymers (CP) of acrylic acid with N-isopropylacrylamide and N-vinylpyrolidine] with BSA-estradiol covalent conjugates at the relatively low concentrations of metal ions in(More)
Aldehyde dextran sulfonate (ADS), a modified oligosaccharide polymer, was used to prepare a new matrix structure for affinity biosensors. The principal difference between the ADS matrix and similar structures developed previously results from presence of two active functional groups in the matrix, namely, aldehyde and sulfonate. These groups perform two(More)
The Cu(2+)-induced complex formation of bovine serum albumin (BSA) with anionic polyelectrolytes (PEs) (polyacrylic acid (PAA), poly(N-isopropylacrylamide) [poly[NIPAAm]], and copolymers of N-isopropylacrylamide (NIPAAm) and acrylic acid) in aqueous solution was studied by a fluorescence technique and high-performance liquid chromatography analysis. The(More)
We have conjugated bovine serum albumin (BSA) to poly(N-isopropylacrylamide-co-acrylic acid) (poly(NIPAAm-AA)) by using water-soluble carbodiimide, and the effects of the bulk mass ratio of protein to polymer (r) on the formation of polymer-protein conjugates have been studied. HPLC, electrophoresis, viscosimetry and fluorescence spectroscopy suggest that(More)
The immunogenic properties of 17beta-estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode,(More)
Cu(2+)-mediated complex formation between polyacrylic acid (PAA) and negatively charged bovine serum albumin (BSA) was studied in neutral water in the presence of Cu2+. Depending on the concentration of Cu2+, the reaction between PAA-Cu2+ complexes and BSA appeared to follow one of two possible paths. At low Cu2+ concentrations (nCu/nAA < 0.15), a further(More)
The immunogenic properties of water soluble (PAA-Cu(2+)-BSA) and colloidal (PAA-Cu(2+)-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity. A comparative study of immunogenic properties of polycomplexes versus(More)
The increasing interest of investigators in polyelectrolyte-metal complexes (PMC) is due, primarily, to the crucial role of metal ions in biological processes [232-234] as well as to the unique capabilities of the PMC proper whose physico-chemical characteristics differ drastically from those of original components, polymer and metal. It is known, for(More)
Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R2, R16)], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water. Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R2, R16) solution at pH 7. A nonrandom distribution(More)