Learn More
The autoimmune regulator (AIRE) protein is a putative transcription regulator with two plant homeodomain-type zinc fingers, a putative DNA-binding domain (SAND), and four nuclear receptor binding LXXLL motifs. We have shown here that in vitro, recombinant AIRE can form homodimers and homotetramers that were also detected in thymic protein extracts.(More)
The aim of this study was to investigate the presence of iNOS in the murine uterus and embryo at implantation. Western blot analysis showed the presence of a 130-kDa band with strong reactivity to anti-iNOS antibody in the pre- and peri-implantation stage uteri. This band was faint in the postimplantation uteri. Immunocytochemical studies showed a heavy(More)
Silkworms show high variability in silk quality and disease resistance. Attempts are on to combine the disease tolerance of multivoltine races and the silk quality of bivoltine races to generate new races with desirable phenotypic traits. We report the identification of a 26.5-kDa protein that is overexpressed in the gut juice of disease-resistant(More)
The distribution of various simple sugar residues over the spermatozoa surfaces of five different mammalian species is characterized and compared. Epididymal maturation of the spermatozoa of all the five species studied exhibited an increase in the amount of N-acetyl-D-glucosamine residues over their acrosomal domains. A complete blockade of sperm-zona(More)
The delta 4-pathway of testosterone biosynthesis in leydig cells, widely believed to proceed through pregnenolone--greater than pregnenedione--greater than progesterone route catalyzed by 5 delta-3 beta-hydroxysteroid dehydrogenase and delta 5-delta 4-isomerase respectively is shown to pass through an alternate pathway mediated by superoxide dismutase and(More)
The emergence of the mammalian blastocysts from their thick glycoprotein investment known as the zona pellucida is an important, but poorly understood, event in embryogenesis. In this paper, we demonstrate that peri-hatching blastocysts generate a considerably high quantum of an active oxyradical species for an extremely short period of time when compared(More)
The autoimmune polyglandular syndrome type 1 (APS1), also known as autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APS1), is a monogenic autosomal disease with recessive inheritance. It is characterized by chronic mucocutaneous candidiasis, multiple autoimmune endocrinopathies, and ectodermal dystrophies. The defective gene responsible for(More)
Mammalian spermatozoa, which like other living cells are susceptible to oxygen toxicity, generate increasing quantities of superoxide radical as epididymal maturation proceeds. This is associated with a concomitant reduction in the activity of superoxide dismutase. A possible involvement of superoxide radicals in bringing about some of the reported(More)
This study was performed to understand the regional distribution of superoxide anion radicals and hydrogen peroxide within the spermatozoa of mice during both normal and altered situations of epididymal maturation. The intracellular O2*- levels were probed employing dichlorodihydrofluorescein diacetate (DDF) as a reporter. The testicular spermatozoa from(More)
Superoxide dismutase, which has been shown to be present in a number of tissues, exhibits cyclic changes during the reproductive cycle of rats. An inverse correlation is seen between the levels of superoxide dismutase and superoxide radical. In immature, pseudopregnant rats, primed with human Chorionic Gonadotropin, lutropin seemed to induce ovarian(More)