Malika Ammam

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In this study we report a new simple process to manufacture a biofuel cell consisting of a glucose oxidase (GOx) based anode and a laccase (LAc) based cathode. The process is based on the electro-deposition of the enzymes, conducting polymer and redox mediators from ultrapure water at a potential of 4V vs. AgCl/Ag. Contrary to the conventional(More)
A highly sensitive and selective glutamate microbiosensor based on polypyrrole (PPy), multiwalled carbon nanotubes (MWCNT) and glutamate oxidase (GluOx) deposited on the transducer platinum electrode (Pt) is described. The sensor consists of a permselective membrane of polypyrrole for the rejection of interferences, followed by a layer of multiwalled carbon(More)
A nitrite sensor based on immobilized Dawson-type tungstophosphate alpha-K(7)[H(4)PW(18)O(62)].18H(2)O (PW(18)) in multilayers of charged polyelectrolyte poly(allylamine hydrochloride) (PAH) on a glassy carbon electrode is described. A nitrite sensor manufactured with 10 layers has a sensitivity of approximately 4 nA/microM nitrite, fast response time(More)
Recent advances in nano-biotechnology have made it possible to realize a great variety of enzyme electrodes suitable for sensing and energy applications. In coating miniaturized electrodes with enzymes, there is no doubt that most of the available deposition processes suffer from the difficulty in depositing uniform and reproducible coatings of the active(More)
The electrophoretic deposition of glucose oxidase from water using asymmetrical alternating voltages is investigated. Using asymmetric voltages, glucose oxidase layers with a thickness of 7 microm could be deposited on a platinum electrode in 20 min time as verified with a microbalance, carbon analysis and scanning electron microscopy. In contrast, if a(More)
In this study, we propose a new strategy to boost the power density of glucose biofuel cells (GBFCs) biocathodes. By combining laccase with catalase enzymes electrophoretically deposited by means of AC electric fields on multiple walled carbon nanotubes modified platinum black and, then stabilized by an outer layer of polypyrrole in the construction of(More)
In this study, we developed an automated strategy to manufacture an enzyme BFC powered by glucose/O(2). The bioanode consists of GOx enzyme and PQQ redox mediator adsorbed over night on MWCNTs then deposited by means of AC-electrophoresis at 30 Hz and 160 V(p-p) and, finally stabilized by electropolymerized polypyrrole. The biocathode is constructed from(More)
Previous work illustrated that glucose oxidase (GOx) could be deposited on conducting substrates using asymmetrical alternating current electrophoretic deposition (AC-EPD) to form thick enzyme layers suitable for the manufacturing of highly active biosensors. Here, we modeled the amperometric response of GOx layers to glucose as a function of the thickness(More)
We report a stainless steel/multi walled carbon nanotubes/polyaniline (SLS/MWCNTs/PANI) capacitor electrode capable of operating in a physiological electrolyte and in serum. The specific capacitance of SLS/MWCNTs/PANI reaches 401 F g(-1) in the physiological electrolyte and 326 F g(-1) in serum. The capacity loss at 6.4 μA mm(-2) over 2000 cycles is 10% in(More)
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